涂层双相陶瓷复合BMSC修复骨缺损的实验观察

目的比较双相陶瓷（Biphasie calcium phosphate，BCP）经低结晶羟基磷灰石（Low crystalline hydroxyapatite，LcHA）涂覆改性后构建的组织工程化骨（LcBCP）与单纯BCP复合骨髓基质干细胞（Bone marrow stromal cells，BMSCs）修复兔桡骨节段性缺损的成骨差异。方法BMSCs复合LcBCP（实验组）修复12只兔左侧桡骨15mm缺损；BMSCs复合BCP（对照组）植入右侧桡骨同样大小缺损，植入后第4、8和12周取材，通过大体形态、组织学、影像学和生物力学检测骨缺损修复效果。结果BMSCs—LcBCP复合物在体内骨缺损处生长良好。X线检测显示实验组连接处骨痂形成，对照组连接处在各个时间点愈合稍差。12周时，实验组骨修复良好，髓腔再通，组织学显示板层骨形成，连接处骨性愈合；对照组连接处尚有较多编织骨形成。实验组和对照组生物力学检测有统计学差异。结论BMSCs—LcBCP复合物可修复兔桡骨节段性缺损，低品态羟基磷灰石涂层有助于增强双相陶瓷的成骨能力。

Experimental Observations on the Repairing of Bone Defects after the Implantation of BMSCs and Biphasic Ceramics Coated with or not with Low Crystalline Hydroxyapatite

Objective To compare the difference of repairing bone defects with autogenous bone marrow stromal cells （BMSCs） and biphasic ceramics （BCP） coated with or not with low crystalline hydroxyapatite （LcHA）. Methods 15 mm segmental radial defects in left and right radii were created In 12 rabbits. Left defects were repaired with cell-scaffold constructs made from induced BMSCs and LcBCP （experimental group）; Right defects were repaired with BMSCs and BCP （control group）. Another 3 rabbits, each with only a defect, were blank group. The engineered bone was evaluated by gross and histological examination, X-ray and biomechanics at 4, 8, 12 weeks post-operation Respectively. Results BMSCs grew well on the LcBCP in vivo. X-ray images showed better callus formation at connection sites in experimental group over time while worse formation at connection sites eventually in control group. At 12 weeks post-operation in experimental group, the defects were well repaired grossly, and reeanalization of the marrow cavities was observed. Histologically, there were bony healing and lamellar bone in experimental group, and more woven bone in control group. Conclusion Rabbits. segmental radial defects can be ultimately repaired by the tissue-engineered bone generated by autogenous osteogenic BMSCs and LcBCP scaffold; Low crystalline hydroxyapatite coatings can enhance bone formation of biphasic ceramics.