富血小板血浆对牙周韧带成纤维细胞在牙骨质表面附着及增殖的影响

目的探讨应用富血小板血浆(PRP)对人牙周韧带成纤维细胞(PDLFs)在健康牙和牙周病患牙牙骨质表面附着和增殖情况的影响。方法采用组织块法培养人牙周膜成纤维细胞,取第四代细胞用于实验。分别制备健康牙和牙周病患牙牙骨质块置于96孔板,附着实验在接种细胞后加入含10%PRP和不含PRP的培养液,4小时后MTT法检测附着细胞数量;增殖实验在接种细胞常规培养24小时,而后加入不含血清的培养液培养48小时,更换为含10%PRP和不含PRP的培养液,24小时后MTT法检测细胞数量;并以扫描电镜观察细胞在牙骨质表面的情况。结果附着实验中10%PRP组附着细胞数量较不含PRP组明显增多;增殖实验中10%PRP组和不含PRP组细胞数量无明显差异。结论10%PRP可促进人PDLFs在健康牙和牙周病患牙牙骨质块上的附着,但对细胞在牙骨质块上的增殖无明显影响。

Effect Of Platelet-rich Plasma On The Attachment And Proliferation Of Periodontal Ligament Fibroblasts On Cementum Slices: An In Vitro Study

Objective The aim of this study was to evaluate the influence of platelet-rich plasma (PRP) on the attachment and proliferation of periodontal ligament fibroblasts (PDLFs) on cementum slices of health teeth and periodontitis affected teeth. Methods Platelet-rich plasma (PRP) was isolated by two-step centrifugation. The 4th passage of human PDLFs were used in this experiment. The cementum slices of health teeth and periodontitis affected teeth were prepared and were put on the 96-well tissue culture plates, and PDLFs were seeded on the cementum slices. Attachment and proliferation of cells were studied by MTT assay. And the status of cells on the cementum slices were observed by electro microscope. Results In the attachment experiment, the PDLFs on cementum slices were significantly increased by addition of 10%PRP, but in the proliferation experiment, there were no significant difference between the experiment group and the control group. Conclusion The attachment of PDLFs on cementum slices were improved by addition of 10%PRP, but 10%PRP had no effect on the proliferation of PDLFs on cementum slices.