氧化应激对多囊卵巢综合征患者体外受精-胚胎移植结局的影响

目的探讨接受体外受精-胚胎移植(IVF-ET)的多囊卵巢综合征(PCOS)患者卵泡液中相关氧化应激指标的水平,并分析氧化应激状态对IVF结局的影响。方法募集进行IVF助孕的PCOS患者25例为PCOS组,因单纯输卵管因素行IVF治疗的患者23例为对照组,收集取卵日清亮卵泡液,检测多种氧化应激相关指标:脂质过氧化产物丙二醛(MDA)、晚期氧化蛋白产物(AOPP)、总抗氧化能力(TAC)、经典的酶类抗氧化物超氧化物歧化酶(SOD)、卵泡液抑制羟自由基(·OH)产生的能力、抗氧化辅酶Q(CoQ10)、还原型及氧化型谷胱甘肽(GSH、GSSG),并分析各项指标与IVF结局的关系。结果与对照组相比,PCOS组卵泡液GSH/GSSG较低,差异有统计学意义(P=0.036);PCOS组宫内妊娠率(35.29%)显著低于对照组(71.43%)(P=0.026);其他指标两组间无显著性差异(P0.05)。结论 PCOS患者卵泡液内存在GSH/GSSG比值异常的氧化应激状态,并可能影响其IVF-ET的临床结局。

Effect of oxidative stress on IVF outcome of patients with polycystic ovary syndrome

Objective： To investigate the effect of oxidative stress on the pregnancy outcome of patients with polycystic ovary syndrome（PCOS）undergoing in vitro fertilization and embryo transfer（IVF-ET）. Methods： The follicular fluid was collected on the egg retrieval day from 25 patients with PCOS （PCOS group）and 23 patients with simple tubal factor（control group）undergoing IVF-ET. The oxidative stress-related indicators, such as malondialdehyde （MDA）, advanced oxidative protein products （AOPP）, total antioxidant capacity（TAC）, superoxide dismutase （SOD）, the ability of follicular fluid to inhibit hydroxyl radical production, coenzyme Q10 （ CoQ10）, reduced glutathione （GSH）, oxidized glutathione （GSSG） were detected. Results： The ratio of GSH/GSSG in the patients with PCOS was significantly lower than that in the control patients（P=0. 036）. The intrauterine pregnacy rate in PCOS group was significantly lower than that in control group （35.29% vs. 71.43%）（P=0. 026）. There were no significant differences in other indicators between the two groups. Conclusions： The abnormal ratio between GSH and GSSG indicates that oxidative stress exists in the follicular fluid of the patients with PCOS,which can influence pregnancy IVF outcome.