PTEN和Caspase-3在骨肉瘤中的表达

目的: 探讨PTEN和Caspase 3的表达在骨肉瘤发生中的可能作用及其临床意义。方法: 用SP免疫组化方法检测PTEN和Caspase 3在 38例骨肉瘤及 20例骨软骨瘤中的表达。结果: PTEN在骨肉瘤中表达阳性率为 55. 3%, 在骨软骨瘤中为 90. 0%, 二者相比差异有非常显著意义 (P=0. 007。Caspase 3在骨肉瘤中表达阳性率为 52. 6%, 在骨软骨瘤中为 85. 0%, 二者相比差异有显著意义 (P=0. 015)。骨肉瘤中PTEN和Caspase 3的表达呈显著正相关 (r=0. 418)。PTEN的低表达与分化程度无关, 与肺转移有关, Caspase 3的低表达与分化程度及肺转移有关。结论: PTEN和Caspase 3与骨肉瘤的发生有关, 且具有相关性, 联合检测PTEN和Caspase 3的表达将对骨肉瘤的早期诊断, 判定恶性程度及预后具有指导意义。

Expression of PTEN and Caspase-3 in osteosarcoma

Objective: To study the expression of PTEN and Caspase 3 in osteosarcoma and their clinical significance.Method:PTEN and Caspase 3 were detected by S P immunohistochemical method in 38 cases of paraffin embedded section of osteosarcoma and 20 of osteochondroma.Result:The positive rate of PTEN in osteosarcoma was 55.3% and 90.0% in osteochondroma ( P =0.007).The positive rate of Caspase 3 in osteosarcoma was 52.6% and 85.0% in osteochondroma ( P =0.015). The expression of PTEN was correlated with Caspase 3 (r=0.418).PTEN was related with pulmonary metastasis instead of differentiation;Caspase 3 was related with both differentiation and pulmonary metastasis.Conclusion: PTEN and Caspase 3 may play an important role in the carcinogenesis of osteosarcoma and are correlated with osteosarcoma. The detection of them might be a guide for the early diagnosis and determinant of malignancy and prognosis in osteosarcoma.