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基质金属蛋白酶家族在骨关节炎软骨组织中表达的研究
引用本文:王玉彬,陈安民,郭风劲,夏玉军.基质金属蛋白酶家族在骨关节炎软骨组织中表达的研究[J].中国矫形外科杂志,2007,15(11):853-855.
作者姓名:王玉彬  陈安民  郭风劲  夏玉军
作者单位:1. 山东省东营市胜利油田中心医院医务科
2. 华中科技大学同济医学院附属同济医院骨科,430030
3. 青岛大学医学院
摘    要:目的]观察骨关节炎关节软骨中MMP-7、MMP-9、MMP-13和TIMP-1的表达,探讨其与软骨退变的关系及可能的作用机制。方法]选取20例因骨关节炎行关节置换的软骨组织,常规HE染色观察其组织学形态,ABC免疫组化法观察关节软骨MMP-7、MMP-9、MMP-13和TIMP-1的表达,2例因意外受伤截肢患者的正常膝关节软骨标本作为对照。统计采用Mann-Whitney U非参数检验及相关分析。结果]骨关节炎关节软骨出现裂隙、纤维化,软骨细胞增多、排列紊乱,并出现大量簇聚软骨细胞和肥大软骨细胞。MMP-7和MMP-13在正常软骨全层均呈低表达,但在退变软骨中的表达则明显增多,光密度值行U检验,两组差异有显著性(P<0.01)。在正常与OA软骨的浅层,MMP-9和TIMP-1的表达无显著性差异(P>0.05);但在深层软骨中,OA软骨MMP-9和TIMP-1的表达较正常软骨明显增多,两组差异有显著性(P<0.01)。结论]MMP-7,13在OA软骨全层表达均多于正常软骨;MMP-9,13仅在OA软骨深层出现过多表达。MMPs与TIMPs的失衡是导致关节软骨发生组织学退变的原因之一。

关 键 词:骨关节炎
文章编号:1005-8478(2007)11-0853-03
收稿时间:2007-03-30
修稿时间:2007-03-302007-04-20

Immunohistochemical expression and significance of MMP-7, MMP-9, MMP-13 and TIMP-1 in osteoarthritis
WANG Yu-bin, CHEN An-min, Guo Feng-jin,et al..Immunohistochemical expression and significance of MMP-7, MMP-9, MMP-13 and TIMP-1 in osteoarthritis[J].The Orthopedic Journal of China,2007,15(11):853-855.
Authors:WANG Yu-bin  CHEN An-min  Guo Feng-jin  
Institution:Tongji Hospital of Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, China
Abstract:Objective]To investigate the relationship between degeneration of cartilage and the expressions of Matrix Metalloproteinase 7(MMP-7),Matrix Metalloproteinase 9(MMP-9),Matrix Metalloproteinase 13(MMP-13)and Tissue Inhibitor of Matrix Metalloproteinase 1(TIMP-1)in osteoarthritis.Method]The histological changes of cartilages by hematoxyllin-eosin staining and immunohistochemical expression of Matrix Metalloproteinase 7(MMP-7),Matrix Metalloproteinase 9(MMP-9),Matrix Metalloproteinase 13(MMP-13)and Tissue Inhibitor of Matrix Metalloproteinase 1(TIMP-1)in osteoarthritis.were studied in 20 osteoarthritis cases and 2 normal controls.All data were statistically analyzed by Mann-Whitney U test and correlation analysis.Result]The osteoarthritis cartilage underwent fibroplasias and tearing.The quantity of chondrocyte increased and the clustered and hypertrophic cells came into being.Little immunostaining of MMP-7 and MMP-13 was observed in normal cartilage,while their expressions increased in degenerated cartilage(P<0.01).Expression of RMP-gand TIMP-1 was the same in normal cartilage(P>0.05),superficial layer of the moderate and end-stage osteoarthritis.However,it significantly increased in deep layer(P<0.01).Conclusion]The abnormal expression of MMP-7,MMP-9,MMP-13 and TIMP-1 was likely to be the pathogenesis of cartilage degeneration.
Keywords:MMP-7  MMP-9  MMP-13  TIMP-1
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