磷酸钙骨水泥对骨形态发生蛋白缓释作用的体外实验研究

目的：研究CPC对BMP的体外缓释作用及其药代动力学，为将CPC与BMP制成复合型生物活性人工骨提供理论依据。方法：以单纯CPC为对照组，以按1g CPC固相配5mg rhBMP-2的配比将CPC与rhBMP－2复合作为实验组，浸入0．01mol/L的磷酸盐缓冲液中，分别在第1、2、3、4、7、14、30、90、180、360d取缓释液，用高效液相色谱仪分析不同时间rhBMP-2缓释量，绘制rhBMP-2缓释曲线，总结药代动力学规律。结果：第1－7d rhBMP-2出现快速释放，释放曲线呈快速上升直线；第7－14d rhBMP－2释放处于平台期；第14－360d rhBMP-2出现缓慢而持久 的释放，释放符合Higuchi方程。结论：CPC能对rhBMP-2有缓慢而持久的体外缓释作用，有望充当rhBMP-2的缓释载体，但缓释速度尚有待提高。

In Vitro Study of CPC's Sustained Release of BMP

Objective:In order to study CPC's sustained release of rhBMP-2 and its kinetics in vitro.Methods:We immerged the CPC/rhBMP-2 compound(1g CPC powder to 5 mg rhBMP-2)into 0.01 mol/L phosphate buffer solution.The solution including rhBMP-2 released from CPC/rhBMP-2 compound was extracted after 1,2,3,4,7,14,30,90,180 and 360 days respectively.The extracted solution was analyzed by Liquid Chromatograph.The release profile of rhBMP-2 from CPC was scaled and its kinetics was assayed.Results:rhBMP-2 had been released rapidly during the 1st to 7th day,slowly and lastingly during the 14th to 360th day,and almost at a platform period during the 7th to 14th day.The release of rhBMP-2 from CPC/rhBMP-2 compound during the 14th to 360th days followed the Higuchi model equation.Conclusion:It can be concluded that CPC showed its characteristics as a sustained delivery system for rhBMP-2 and may be used as a novel sustained release carrier for rhBMP-2 in clinic.