单核细胞趋化蛋白-1基因多态性对脊柱结核易感性的影响

目的]分析趋化因子单核细胞趋化蛋白-1(MCP-1)启动子区362基因型频率和等位基因多态性与中国湖南省汉族人群脊柱结核易感性的关系.方法]选取2007年12月～2010年12月本院收治的湖南汉族新发脊柱结核患者166例(病例组)及健康志愿者200例(对照组),应用聚合酶链式反应(PCR)和DNA直接测序法检测两组患者MCP -1 - 362位点的多态性,并进行MCP -1基因分型.结果]MCP -1 - GG、GC、CC三种基因型在病例组和对照组中的分布频率分别为18.1％、42.8％、39.2％和25.5％、48.0％、26.5％.两组组间比较有显著性差异(P＜0.05),其中MCP -1 - CC基因型在病例组中的分布频率明显高于对照组,比值比(odds ratio,OR)为1.478,其95％可信区间(confidence interval,CI)为1.096～1.992.结论]在湖南汉族人群中,MCP -1 - 362位点CC基因型可能与脊柱结核的易感性相关.

Influence of monocyte chemoattactant protein-1 gene polymorphism on susceptibility of spinal tuberculosis

Objective]To investigate the association between monocyte chemoattactant protein-1(MCP-1) gene polymorphism and the susceptibility to spinal tuberculosis in Han people of Hunan province. Methods]Totally 166 patients with spinal tuberculosis(case group) and 200 healthy volunteers(control group) were recruited in our hospital from December 2007 to December 2010.The MCP-1-362 genotype were detected by polymerase chain reaction and sanger DNA sequencing technology. Results]The genotype frequencies of GG,GC,CC were 18.1%,42.8%,39.2% in case group,and 25.5%,48.0%,26.5% in control group.There were significant differences between two groups(P<0.05).The MCP-1-362 CC genotype were overexpressed in case group,the odds ratio was 1.478(95% CI: 1.096-1.992). Conclusion]The MCP-1-362 CC genotype might be associated with susceptibility to spinal tuberculosis in Han people of Hunan province.