血管内皮生长因子基因多态性与糖尿病肾病的相关性研究

目的 探讨血管内皮生长因子(VEGF)-634G／C基因多态性与糖尿病肾病(DN)的关系。方法 运用PCR-限制性多态性片段长度(RFLP)技术检测98例健康对照者和216例2型糖尿病患者其中DN患者104例，单纯2型糖尿病(DM)患者112例]的基因型，比较各组的基因型和等位基因频率。结果 (1)CC基因型者血清VEGFT水平高于CG及GG型者；(2)DN组CC基因型和C等位基因频率显著高于DM组和正常对照组；(3)与GG型和CG型组相比，CC型组DN的发生率明显上升；(4)Logistic回归分析显示VEGF、VEGF基因多态性、收缩压(SBP)、HbA1c、LDL-C、体重指数(BMI)是DN的危险因素。结论 VEGF-63gG／C多态性与2型DM伴发肾病的发生有关，C等位基因可能是DN的易感基因。

Correlation of vascular endothelial growth factor gene polymorphism to diabetic nephropathy in type 2 diabetes mellitus

Objective To investigate the correlation of vascular endothelial growth factor (VEGF)-634G/C gene polymorphism to diabetic nephropathy in type 2 diabetes mellitus. Methods VEGF genotype was determined by PCR-RFLP in 98 healthy controls and 216 patients with type 2 diabetes including 104 cases without nephropathy and 112 cases with nephropathy. VEGF genotype and allele frequencies were compared among these groups.Results (1)Individuals with CC genotype had higher serum VEGF level than those with CG and GG groups. (2)The genotype CC distribution and C allele frequency in diabetic nephropathy were significantly higher than those in cases without nephropathy group and in controls. (3)Compared with genotype GG and CG class, the morbidity of diabetic nephropathy in genotype CC class was significantly increased. (4) Logistic regression analysis revealed that serum VEGF level and its CC genotype, SBP, HbA1C, LDL-C and BMI were important risk factors of type 2 diabetic nephropathy. Conclusions VEGF -634G/C polymorphism is associated with the development of type 2 diabetic nephropathy. C allele is probably a susceptible gene of type 2 diabetic nephropathy.