梗阻性肾病大鼠肾组织periostin的表达及意义

目的 研究梗阻性肾病大鼠肾组织periostin表达的变化及其与肾间质纤维化的相关性.方法 18只SD雄性大鼠按随机数字法分成3组:假手术组、模型组和贝那普利组,每组6只.用单侧输尿管结扎法建立梗阻性肾病大鼠模型.RT-PCR检测各组大鼠肾组织periostin和转化生长因子(TGF) β1的mRNA表达；ELISA检测各组大鼠肾组织periostin、血管紧张素Ⅱ( AngⅡ)、TGF- β1的蛋白水平；HE及Masson染色观察肾间质变化；免疫组化检测肾组织Ⅰ型胶原蛋白.结果 与假手术组比较,模型组大鼠肾组织periostin、TGF-β1、AngⅡ蛋白表达显著升高(均P＜0.05).与模型组比较,贝那普利组上述蛋白表达显著降低,差异有统计学意义(均P＜0.05).与假手术组比较,模型组大鼠肾组织periostin和TGF- β1的mRNA表达显著上调,差异有统计学意义(均P＜0.05),而贝那普利组periostin和TGF- β1的mRNA表达则显著下调(均P＜0.05).肾组织periostin蛋白表达与AngⅡ、TGF-β1和Ⅰ型胶原的蛋白表达以及肾间质纤维化积分均呈正相关(r值分别为0.652、0.781、0.776和0.825,均P＜0.05).结论 梗阻性肾病大鼠肾组织periostin呈高表达,其可能参与了梗阻性肾病肾间质纤维化进程.

Expression of periostin in the kidneys of rats with obstructive nephropathy and its significance

Objective To investigate the expression of periostin in the kidneys of rats with obstructive nephropathy and its relevance to renal interstitial fibrosis. Methods Eighteen male adult SD rats were randomly divided into sham group, model group and benazepril group (6 in each group). Unilateral ureteral obstruction (UUO) model was induced by ligating the left ureter of rats. RT-PCR was used to detect the mRNA expressions of periostin and TGF-β1, and ELISA to detect the protein expression of periostin, AngⅡ, and TGF-β1 in kidney tissue. Pathological changes of renal tissue were observed by HE and Masson staining. The protein expression of collagen Ⅰ(ColⅠ) in kidney tissue was examined by immunohistochemical staining. Results The mRNA expressions of periostin and TGF-β1 in model group increased markedly as compared with sham group (all P<0.05), and benazepril could decrease these mRNA expressions (all P<0.05). The protein expressions of periostin, AngⅡ and TGF-β1 in kidney tissue were significantly increased in model group as compared with sham group (all P<0.05), and benazepril could decrease these protein expressions (all P<0.05). The expression of periostin in kidney tissue was positively correlated with the expressions of AngⅡ，TGF-β1 and ColⅠ, as well as the degree of renal interstitial fibrosis (r=0.652, 0.781, 0.776 and 0.825 respectively, all P<0.05). Conclusion Periostin expression is up-regulated in kidney tissue of rats with obstructive nephropathy, which is associated to the over-deposition of extracellular matrix (ECM) in the kidneys of UUO rats.