| CDH13表达下调对膀胱癌细胞增殖、克隆形成及PI3K/Akt表达的影响 |
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| 引用本文： | 林英立,李艳丽,戚景光,马建国,李文平,孙光.CDH13表达下调对膀胱癌细胞增殖、克隆形成及PI3K/Akt表达的影响[J].国际泌尿系统杂志,2017,37(1). |
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| 作者姓名： | 林英立 李艳丽 戚景光 马建国 李文平 孙光 |
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| 作者单位： | 1. 徐州 徐州市肿瘤医院泌尿外科,江苏,221005;2. 徐州 徐州市肿瘤医院科教科,江苏,221005;3. 天津医科大学第二医院泌尿外科,天津,300211;4. 石家庄 河北医科大学第三医院泌尿外科,河北,050051 |
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| 基金项目： | Young Medical Talents Project of Xuzhou City，Science and Technology Project of Xuzhou City，Jiangsu University Clinical Science and Technology Development Fund Project，Jiangsu Province "Six Talent Peaks" Funded Project，Youth Scientific Research Project of Jiangsu Provincial Health and Family Planning Commission(Q201514)徐州市医学青年后备人才项目，徐州市科技计划项目，江苏大学临床科技发展基金项目，江苏省“六大人才高峰”自助项目，江苏省卫计委青年科研课题项目 |
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| 摘 要： | 目的 研究膀胱癌5637细胞中CDHI3表达下调后对细胞增殖、克隆形成及PI3K/Akt表达的影响.方法 应用RNA干扰技术抑制5637细胞中CDH13的表达,细胞克隆形成实验检测细胞克隆形成率,MTT法检测细胞增殖能力,Western Blot检测CDH13、PI3K及Akt的表达水平.结果 膀胱癌5637细胞中CDH13表达下调后细胞克隆形成及增殖能力增强,PI3K及Akt的表达增加.结论 膀胱癌细胞中CDH13表达下调后可能通过激活PI3K/Akt通路促进肿瘤细胞增殖和克隆形成.
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| 关 键 词： | 膀胱肿瘤 细胞增殖 RNA |
The influence of CDH13 down-regulation on the proliferation,clone and PI3K/Akt expression of bladder cancer cell |
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| Abstract: | Objectives To investigate the influence of CDH13 down-regulation on the proliferation,clone and PI3K/Akt expression of bladder cancer cell.Methods RNA interference was used to inhibit CDH13 expression in 5637 cells,clone formation assay was used to examine cell clone formation,MTT was used to evaluate cell proliferation,the expression of CDH13,PI3K and Akt was exa-mined by Western Blot.Results The cell clone,proliferation and PI3K/Akt expression were increased after the down-regulation of CDHl3.Conclusions Down-regulation of CDH13 expression in bladder cancer cell promotes tumor cell clone and proliferation by PI3K/Akt pathway. |
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| Keywords: | Bladder Neoplasms Cell Proliferation RNA |
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