加味启膈散抑制细胞增殖治疗Barrett食管作用机制研究

目的:观察加味启膈散对Barrett食管(BE)模型大鼠PCNA、Ki-67蛋白表达的影响。方法:SPF级成年健康Wistar大鼠72只,雌雄各半,常规适应性喂养7天后,随机分为6组:正常组、模型组、全方组、行气活血组、理气化痰组、化痰祛瘀组,每组12只。采用改良后的"食管十二指肠端侧吻合加铁剂术"进行BE造模。造模31周后,正常组、模型组大鼠给予生理盐水灌胃,各治疗组分别给予相应药液灌胃,连续给药14天后,处死动物留取吻合口食管组织,采用免疫组化法检测PCNA、Ki-67蛋白表达情况。结果:与正常组比,模型组大鼠Ki-67表达显著增强;全方组Ki-67表达低于模型组(P<0.05);理气化痰组和行气活血组Ki-67表达水平高于全方组(P<0.05),化痰祛瘀组Ki-67表达与全方组差异无统计学意义(P>0.05)。模型组PCNA表达水平较正常组显著增强(P<0.05);全方组PCNA表达水平显著低于模型组(P<0.05);理气化痰组和行气活血组PCNA表达均高于全方组,差异有统计学意义(P<0.05),化痰祛瘀组与全方组PCNA表达差异无统计学意义(P>0.05)。结论:加味启膈散抑制细胞增殖治疗Barrett食管,其机制与抑制PCNA、Ki-67蛋白表达有关。

Research on Mechanism of Jiawei Qige San Inhibits Cell Proliferation in the Treatment of Barrett' s Esophagus Action

Objective To observe the effect of Qige San onthe PCNA andKi-67 protein expression of BE model rat.Methods Seventy-two clean adult Wistar rats,male and female,were randomly divided into 6 groups,12 rats in each group,which were normal control group,model control group,and traditional Chinese medicine group.The whole traditional Chinese medicine group divided into the Chinese medicine Qi and activating blood group,the Chinese medicine qi stagnation group,the Chinese medicine phlegm group.Except the normal group,the other five groups were modeled on BE.After successful modeling for 31 weeks,rats in the normal control group and the model control group were intragastrically administered with normal saline from the beginning of the 32nd week.Rats in each treatment group were given the corresponding liquid weight.After 14 days of treatment,the animals in each group were sacrificed for index detection.Results For the expression of Ki-67,compared with the normal group,the expression levels of Ki-67 in the model group,the Chinese medicine Qi stagnation group,the Chinese medicine qi stagnation group and the Chinese medicine phlegm group were higher than those of the normal group.The differences were statistically significant(P<0.05).Compared with the model group,the expression of Ki-67 in the whole group was lower than that of the model group,which the difference was statistically significant(P<0.05).Compared with the whole group,the expression of Ki-67 in the Chinese medicine Liqi Huatan group and the Chinese medicine Xingqi Huoxue group was higher than those of the whole group and the difference was statistically significant(P<0.05).Compared with the normal group,the expression of PCNA in the model group,the Chinese medicine qi and blood group and the Chinese medicine phlegm group were higher than that in the normal group and the difference was statistically significant.The significance of the study was P<0.05.The difference between the whole group and the normal group was not statistically significant(P>0.05).Compared with the model group,the PCNA expression level of the whole group was lower than that of the model group.The difference was statistically significant(P<0.05).Compared with the whole group,the expression of PCNA in the Chinese medicine Liqi Huatan group and the Chinese medicine Xingqi Huoxue group was higher than that in the whole group,the difference was statistically significant(P<0.05).The expression of PCNA in the Chinese medicine phlegm group was higher than that in the whole group,but the difference was not statistically significant(P>0.05).For the Chinese medicine qi sputum group,Chinese medicine qi and blood circulation group,there were no significant differences between the Chinese medicine and phlegm groups(P>0.05).Conclusion Jiawei QigeSan inhibits cell proliferation and treats Barrett’s esophagus.Its mechanism is related to the inhibition of PCNA and Ki-67 protein expression.