蒲公英甾醇调控膀胱癌T24 细胞迁移与侵袭能力的作用及机制研究

目的:探索蒲公英甾醇对膀胱癌T24细胞迁移与侵袭能力的作用及机制。方法:体外培养膀胱癌T24细胞,在其中加入不同浓度(10、20、40μg/mL)蒲公英甾醇进行干预。采用划痕实验和Transwell小室法分别观察膀胱癌细胞迁移能力及侵袭能力;Western blotting实验考察细胞中SDF-1/CXCR4信号通路相关蛋白的变化;ELISA试剂盒检测细胞上清中基质金属蛋白酶(MMP)-2/9的水平。结果:蒲公英甾醇可抑制膀胱癌T24细胞的迁移与侵袭能力(P<0.05,P<0.01),降低细胞分泌MMP-2及MMP-9的能力(均P<0.05,P<0.01)。同时蒲公英甾醇能有效抑制膀胱癌T24细胞内基质细胞衍生因子-1(SDF-1)、CXCR4、磷酸化蛋白激酶B(p-Akt)及磷酸化哺乳动物雷帕霉素靶蛋白(p-mTOR)的表达水平(P<0.05,P<0.01)。结论:蒲公英甾醇通过干扰SDF-1/CXCR4信号通过,下调Akt/mTOR的传导,削弱了细胞分泌MMP-2及MMP-9的能力,从而有效抑制膀胱癌T24细胞的迁移与侵袭。

Effect and Mechanism of Taraxasterolon Migration and Invasion of T24 Bladder Cancer Cells CHEN

Objective To explore the effect and mechanism of taraxasterol on T24 bladder cancer cell migration and invasion.Methods T24 bladder cancer cells were cultured in vitro and then different concentrations(10,20,40μg/mL)of taraxasterol were added.The migration and invasion abilities of bladder cancer cells were observed by the scratch test and the transwell assay.Western blotting assay was performed to investigate the changes of SDF-1/CXCR4 signaling related protein expressions.The level of MMP-2/9 in cell supernatant was detected by ELISA method.Results Taraxasterol inhibited the migration and invasion of T24 bladder cancer cells(P<0.05,P<0.01)and decreased the ability of cells to secrete MMP-2 and MMP-9(P<0.05,P<0.01).Meanwhile,taraxasterolcan effectively inhibit the expression levels of SDF-1,CXCR4,p-Akt and p-mTOR in T24 cells(P<0.05,P<0.01).Conclusion Taraxasterolcould interfere with SDF-1/CXCR4 signal,down-regulate the Akt/mTOR conduction and weaken the ability of T24 cells to secrete MMP-2 and MMP-9.Finally,taraxasteroleffectively inhibit the migration and invasion of T24 bladder cancer cells.