| 富含血小板血浆双相接种法构建组织工程骨 |
| |
| 引用本文： | 雷华,肖苒,曹蕊,吕晓岩,归来.富含血小板血浆双相接种法构建组织工程骨[J].中国美容医学,2011,20(3):402-407. |
| |
| 作者姓名： | 雷华 肖苒 曹蕊 吕晓岩 归来 |
| |
| 作者单位： | 北京协和医学院·中国医学科学院·整形外科医院整形六科,北京,100144 |
| |
| 摘 要： | 目的：传统的细胞接种法接种效率低,影响组织工程骨的成骨质量。本研究预探讨富含血小板血浆双相接种法是否可以提高细胞接种效率、优化组织工程骨的构建。方法：分别用富含血小板血浆、乏血小板血浆重悬BMSCs,灌注于三维多孔PLGA支架中,凝血酶促凝,体外培养。通过扫描电镜观察支架表面的细胞分布;通过检测支架内DNA含量、ALP含量、成骨基因表达情况分析细胞的接种效率、增殖和分化情况,并与传统的接种方法进行比较。结果：富含血小板血浆和乏血小板血浆双相接种法均能提高细胞与三维支架的结合效率;富含血小板血浆还可以促进支架内细胞的增殖,同时不影响细胞的分化。结论：双相接种法可以提高细胞的接种效率;与普通双相接种法相比,富含血小板血浆双相接种法可以进一步优化组织工程骨的构建。
|
| 关 键 词： | 富含血小板血浆 细胞接种 骨组织工程 |
Constructing tissue engineering bone by a biphasic seeding strategy with platelet-rich plasma |
| |
| LEI Hua,XIAO Ran,CAO Rui,LV Xiao-yan,GUI Lai.Constructing tissue engineering bone by a biphasic seeding strategy with platelet-rich plasma[J].Chinese Journal of Aesthetic Medicine,2011,20(3):402-407. |
| |
| Authors: | LEI Hua XIAO Ran CAO Rui LV Xiao-yan GUI Lai |
| |
| Institution: | LEI Hua,XIAO Ran,CAO Rui,LV Xiao-yan,GUI Lai(The Sixth Department,Plastic Surgery Hospital,Peking Union Medical College,China Academy of Medical Sciences,Peking,Beijing 100144,China) |
| |
| Abstract: | Objective The conventional static seeding is not efficient in constructing tissue engineering bone,which impacts the quality of osteogenesis.This study wants to prove if a biphasic seeding strategy with platelet-rich plasma(PRP) can improve the conventional static seeding.Methods The BMSCs suspension in PRP or platelet-poor plasma(PPP) was infiltrated in 3D PLGA scaffolds,clotted by thrombin and cultured for different days.The cell seeding efficiency was evaluated by SEM and by measuring initial DNA content... |
| |
| Keywords: | platelet-rich plasma(PRP) cell seeding bone tissue engineering |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
