骨髓间充质干细胞复合组织工程化脱细胞真皮基质构建组织工程皮肤

目的：体外培养扩增SD大鼠骨髓间充质干细胞（bone marrow mesenchymal stem cells,BMSCs），复合组织工程化脱细胞真皮基质构建组织工程皮肤，为进一步临床应用奠定基础。方法：将SD大鼠骨髓间充质干细胞进行体外培养扩增后，以生长状态良好的骨髓间充质干细胞接种于制备好的组织工程化脱细胞真皮支架上，进行体外联合培养，构建组织工程皮肤。观察细胞生长情况及组织工程皮肤结构。结果：体外培养的SD大鼠骨髓间充质干细胞生长良好，传代扩增容易，组织工程化脱细胞真皮基质去细胞完全，骨髓间充质干细胞在脱细胞真皮基质中生长良好，可体外构建组织工程皮肤。结论：利用体外扩增培养的骨髓间充质干细胞及制备的组织工程化脱细胞真皮基质可以体外联合构建组织工程皮肤。

Construction of tissue-engineered skin with BMSCs and tissue-engineered acellular dermal matrix

Objective To culture and proliferate bone marrow mesenchymal stem cells (BMSCs) from bone marrow of SD rat in vitro, and to construct tissue-engineered skin with the cultured cells as well as tissue-engineered acellular dermal matrix. Methods BMSCs were separated from bone marrow of SD rat, and purified by combining density gradient centrifugation with adhering method. The cultured cells in great shape were seeded onto the surface of acellular dermal matrix. It was cultured absolutely inside liquid medium for about 12d. In this way, tissue-engineered skin was expected to be constructed in vitro. The cultured cells were observed growing and proliferating well in vitro. Results The cultured cells were observed growing and proliferating well in vitro. With the cells cultured in the acellular dermal matrix,tissue-engineered skin could be construted in virto. Conclusion Constuction of tissue-engineered skin with BMSCs as well as tissue-engineered acellular dermal matrix in vitro is feasible.