抑癌基因PTEN在乳腺浸润性导管癌中的表达及临床意义

目的研究乳腺浸润性导管癌中PTEN蛋白表达与乳腺癌不同的临床病理因素、TNM分期的关系及其临床意义。方法采用免疫组织化学SP法,对32例乳腺浸润性导管癌组织中PTEN蛋白的表达水平进行检测。结果正常乳腺组织中PTEN表达阳性,32例乳腺癌中有13例PTEN阴性表达(41%),其中8例雌激素受体(ER)、孕激素受体(PR)阴性(61.5%),而19例PTEN阳性表达标本中仅4例ER、PR均为阴性(21.1%)。有腋淋巴结转移的乳腺浸润性导管癌组织中PTEN表达水平显著低于无腋淋巴结转移的乳腺浸润性导管癌组织(P<0.001);ER阴性者PTEN表达水平明显低于ER阳性者(P<0.05),PTEN表达水平与患者年龄、肿瘤大小、孕激素水平及临床分期无关,但随临床分期的增高,PTEN表达水平呈下降趋势。结论乳腺癌组织中PTEN蛋白失表达与腋淋巴结转移及雌激素阴性水平密切相关。

Expression and clinical significance of PTEN gene in breast cancer

Objective To investigative the correlation between the expression level of PTEN with its various clinic pathological types,TNM stage of breast cancer.Methods The expression of PTEN in 32 cases of breast cancer was detected by immunohistochemistry S-P method.Results Positive expression of PTEN was found in all normal breast tissues.Of the 13 cases (41 %) of breast cancer negative for the PTEN protein expression,8 cases (61.5 %) were negative for estrogen and progesterone receptor.While in 19 cases positive for the PTEN protein expression,one 4 cases (21.1 %) were positive for the PTEN protein expression.The expression level of PTEN in mammary infiltrative tumor with axillary lymph node metastasis was remarkably lower than that without axillary lymph node metastasis (P<0.001).The expression level of PTEN in the patients negative for ER was significantly lower than in those positive for ER (P<0.05).Expression level of PTEN was not related to age,tumor size,progesterone receptor and clinical stage.However,as increasing of clinical stage,the expression level of PTEN was declined.Conclusion The loss expression of PTEN protein is associated with axillary lymph node metastasis and steroid receptor hormonal loss.