人骨肉瘤外周血T淋巴细胞亚群和自然杀伤细胞免疫变化的研究

目的研究人骨肉瘤患者外周血T淋巴细胞亚群和自然杀伤细胞(NK细胞)的变化。方法采用流式细胞术对42例骨肉瘤、41例骨髓炎、34例健康者的外周血CD4 、CD8 、CD4 、CD4 /CD8 、CD3 、CD16 56均显著性低于骨髓炎患者和健康对照组(P<0.01),而CD8 则升高(P<0.05)。骨髓炎患者外周血CD4 、CD4 /CD8 、CD3 较健康人降低,CD8 则升高,但差异均无统计学意义(P>0.05),而CD16 56与健康对照组相比降低(P<0.05)。结论骨肉瘤患者细胞免疫功能明显降低;骨肉瘤患者的免疫抑制远较骨髓炎的强;骨肉瘤患者NK细胞的降低较骨髓炎患者明显多。流式细胞术可作为检测骨肉瘤患者免疫功能的有效方法。

Changes of T lymphocyte subsets and NK cells in peripheral blood of the patients with osteosarcoma

Objective To investigate the immune function of the cells in the peripheral blood of the patients with osteosarcoma.Methods Using flow cytometry,CD_4~ ,CD_3~ and CD_8~ T lymphocyte subsets and NK cells of peripheral blood were quantitatively measured in 42 patients with osteosarcoma,41 patients with osteomyelitis and 34 normal controls.Results The CD_4~ and CD_3~ T cells,helper/suppressor radio(CD_4~ /CD_8~ ),and CD_(56 16)were significant1y lower(P<0.01) and CD_8~ T cells were significantly higher(P<0.05) in patients with osteosarcoma than the patients with osteomyelitis and in normal controls.There was no significant difference in the CD_4~ ,CD_3~ and CD_8~ T cells and helper/suppressor radio(CD_4~ /CD_8~ ) between the patients with osteomyelitis and the normal controls(P>0.05),but CD_(56 16) was lowered in osteomyelitis patients as compared with the normal controls(0.01