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| 靶向CTNNB1的shRNA抑制结肠癌SW480细胞增殖的研究 | |
| 引用本文： | 黄文生,王天宝,伍虹,钟诗龙,谭敏.靶向CTNNB1的shRNA抑制结肠癌SW480细胞增殖的研究[J].中华普通外科学文献（电子版）,2011,5(2):12-15. |
| 作者姓名： | 黄文生 王天宝 伍虹 钟诗龙 谭敏 |
| 作者单位： | 1. 中山大学附属第一医院外科,广州,510080 2. 中山大学孙逸仙纪念医院口腔科 3. 广东省人民医院医学研究中心 |
| 基金项目： | 广东省科技厅科研基金资助项目 |
| 摘 要： | 目的探讨靶向CTNNB1的shRNA对人结肠癌SW480细胞的基因下调效应和细胞增殖的抑制作用.方法构建靶向CTNNB1的shRNA载体质粒,然后转染入结肠癌SW480细胞.用RT-PCR和Western blotting方法检测CTNNB1的mRNA和蛋白的表达情况.MTT实验评价转染后各组细胞的增殖情况,并用流式细胞仪检测各组细胞的周期分布和凋亡情况.结果靶向CTNNB1的shRNA能够明显下调CTNNB1 mRNA和蛋白质表达（P＜0.05）,其抑制率分别是43.87%和45.16%.MTT实验提示CTN组细胞在转染后呈现随着时间延长而进行性增殖抑制的现象.在转染后72 h,CTN组的细胞存活率为46.4%,与空白对照组相比有显著性降低（P＜0.05）.而流式细胞仪显示CTN组细胞有明显G0/G1周期阻滞和凋亡率增高（P＜0.05）.结论靶向CTNNB1的特异性shRNA对结肠癌SW480细胞具有下调CTNNB1基因表达,促进细胞凋亡并且显著抑制细胞增殖的效应. |
| 关 键 词： | CTNNB1 结肠癌 RNA干扰 ShRNA |
ShRNA targeting against CTNNB1 inhibits growth of human colon cancer SW480 cells |
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| HUANG Wen-sheng,WANG Tian-bao,WU Hong,TAN Min.Surgery.ShRNA targeting against CTNNB1 inhibits growth of human colon cancer SW480 cells[J].Chinese Journal of General Surgery(Electronic Version),2011,5(2):12-15. | |
| Authors: | HUANG Wen-sheng WANG Tian-bao WU Hong TAN MinSurgery |
| Institution: | HUANG Wen-sheng,WANG Tian-bao,WU Hong,TAN Min.Surgery Department,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China |
| Abstract: | Objective To observe the inhibitory effect of CTNNB1 gene expression and cell proliferation of the human colon cancer cell line SW480 caused by CTNNB1-targeted ShRNA.Methods The shRNA against CTNNB1 was constructed and transfected into SW480 cells.The down-regulations of CTNNB1 expressions were detected by RT-PCR and Western blotting analysis.The cell proliferation was determined by soft agar colony formation assay.The effect of these shRNAs on cell cycle distribution and apoptosis was examined by flow cyto... |
| Keywords: | CTNNB1 ShRNA |
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