白茅苷对去卵巢大鼠骨髓间充质干细胞功能和骨量的影响

目的观察白茅苷治疗去卵巢大鼠骨髓间充质干细胞功能和骨量的影响并初步探索可能机制。方法通过双侧去卵巢建立骨质疏松大鼠模型;随后随机分为假手术组(Sham)、去卵巢组(OVX)以及白茅苷组(BMG),每组10只;其中BMG组去卵巢大鼠每天给予白茅苷(20 mg/kg)灌胃治疗;待12周治疗结束后分离培养各组大鼠骨髓间充质干细胞(BMSCs),使用碱性磷酸酶(ALP)和茜素红(ARS)染色并使用蛋白质印迹检测BMP-2、Runx2、OPN、OCN、ALP和Col1蛋白表达;进一步使用Micro-CT和骨生物力学检测观察治疗效果。结果 OVX组大鼠BMSCs向成骨细胞分化后ALP和ARS染色阳性面积以及BMP-2、Runx2、OPN、OCN、ALP和Col1表达较Sham组明显降低(P0.05);而经过BMG治疗,BMG组大鼠BMSCs向成骨细胞分化后ALP和ARS染色阳性面积以及BMP-2、Runx2、OPN、OCN、ALP和Col1表达较OVX组明显增加(P0.05)。OVX组股骨最大载荷和弹性模量、BMD、BV/TV、Tb.N和Tb.Th较Sham组明显降低,而Tb.Sp则明显升高(P0.05)。BMG组左侧股骨最大载荷和弹性模量、BMD、BV/TV、Tb.N和Tb.Th均明显高于OVX组(P0.05),而Tb.Sp明显低于OVX组(P0.05)。结论白茅苷通过促进BMSCs诱导成骨分化来减少去卵巢大鼠骨骨密度、骨量和骨强度下降。

The effect of imperatorin on bone marrow mesenchymal stem cell function and bone mass in ovariectomized rats

Objective To observe the effect of imperatorin treatment on bone marrow mesenchymal stem cell function and bone mass in ovariectomized rats and to explore the possible mechanisms. Methods Osteoporosis rat model was established with bilateral ovariectomy. Rats were then randomly divided into sham operation group (Sham), ovariectomy group (OVX), and imperatorin group (BGM), with 10 rats in each group. Rats in BGM group ovariectomy received imperatorin (20 mg/kg) by gavage every day. After 12-week treatment, bone marrow mesenchymal stem cells (BMSCs) of each group were isolated and cultured. Alkaline phosphatase (ALP) and Alizarin red (ARS) staining and Western blotting were used to detect the protein expressions of BMP-2, Runx2, OPN, OCN, ALP, and Col1. Micro-CT and bone biomechanics were used to observe the treatment effect. Results The positive area of ALP and ARS staining and the expression of BMP-2, Runx2, OPN, OCN, ALP, and Col1 in BMSCs differentiated into osteoblasts in the OVX group were significantly lower than those in the Sham group (P<0.05). After imperatorin treatment, the positive area of ALP and ARS staining and the expression of BMP-2, Runx2, OPN, OCN, ALP, and Col1 in BMSCs differentiated into osteoblasts significantly increased compared to those in OVX group (P<0.05). The maximum load and elastic modulus, BMD, BV/TV, Tb.N, and Tb.Th of the femur in OVX group were significantly lower than those in Sham group, while Tb.Sp was significantly increased (P<0.05). The maximum load and elastic modulus, BMD, BV/TV, Tb.N, and Tb.Th of the left femur in the imperatorin group were significantly higher than those in the OVX group (P<0.05), while Tb.Sp was significantly lower than that in the OVX group (P<0.05). Conclusion Imperatorin reduces the decrease of bone mineral density, bone mass, and bone strength in ovariectomized rats by promoting BMSCs to induce osteogenic differentiation.