新伐他汀体外对破骨细胞性骨吸收及大鼠骨代谢的影响

目的探讨新伐他汀体外对破骨细胞骨吸收功能的作用及其大鼠骨代谢的影响.方法采用体外成熟破骨细胞和大鼠颅盖骨培养体系,检测新伐他汀作用7 d后破骨细胞骨吸收陷窝和培养上清钙的变化;检测大鼠颅盖骨培养上清碱磷酶和钙含量,组织学观察颅盖骨形态学变化.结果新伐他汀体外可明显抑制破骨细胞骨吸收陷窝的形成及培养上清钙的释放,新伐他汀体外可增强大鼠颅盖骨培养上清碱磷酶的活性,组织学观察到新伐他汀使大鼠颅盖骨矿化增强.结论新伐他汀体外不仅可促进大鼠颅盖骨的成骨活性,并且可明显抑制破骨细胞骨吸收功能,对骨质疏松有重要的防治作用.

Effect of the Simvastatin on the osteoclastic resorption and bone anabolism with murine calvarial organ culture in vitro

Objective To study the effect of Simvastatin in the osteoclastic resorption and murine bone anabolism in vitro.Methods The bone resorption activities of the osteoclast were evaluated after treatment of Simvastatin for 7 days in vitro;the concentration of Ca~ 2 in the superant was also detected by atomic absorption spectrometer.The concentrations of ALP and Ca~ 2 of the superant in murine calvarial organ culture were detected.The histology of calvaria of was observed.Results Simvastatin greatly inhibited the osteoclastic bone resorption in vitro and reduced the release of Ca~ 2 .Simvastatin increases the ALP activities and bone mineralization of murines calvarial organ culture in vitro.Conclusions Simvastatin may inhibit the osteoclastic resorption and promote osteoblast differentiation and bone mineralization in vitro,thus play an important role in the prevention and treatment of osteoporosis.