绝经后骨质疏松症肾阴虚证关联LincRNA uc431 +的表达研究

目的探讨lincRNA uc431+与绝经后骨质疏松症(postmenopausal osteoporosis,POP)肾阴虚证的关系及其二级结构特征。方法在前期研究证实了CLCF1是POP肾阴虚证的重要关联基因,并在血液lncRNA芯片检测中筛选了POP肾阴虚证的特异lincRNAs,本文采用blat软件对靶基因为CLCF1的lncRNA进行分析;采用分析软件RNAfold对lncRNA及其二级结构进行预测;随机选择绝经后骨质疏松症患者,中医辨证分型为肾阴虚证组25例,健康绝经后妇女25例设为正常对照组。用定量PCR技术检测绝经后骨质疏松症肾阴虚证组、对照组外周血lincRNA uc431+及CLCF1的表达水平。结果 blat软件预测lincRNA uc431+的靶基因为CLCF1,相关系数为-0.8192(P=0.0069);RNAfold软件预测发现lincRNA uc431+有多个茎环结构;实时荧光定量PCR结果表明,POP肾阴虚证组CLCF1 mRNA表达水平明显低于对照组(P0.01),lincRNA uc431+在POP肾阴虚证组中表达出现降低,与对照组相比,差异具有统计学意义(P0.01)。结论 lincRNA uc431+的表达下调可能与绝经后骨质疏松症肾阴虚证相关联。

Study on the expression of lincRNA uc431 + in postmenopausal osteoporosis patients with kidney-yin deficiency syndrome

Objective To investigate the expression of lincRNA uc431 + in postmenopausal osteoporosis patients with kidney Yin deficiency and it secondary structural characteristics. Methods In previous studies, we had confirmed that CLCF1 is correlated with postmenopausal osteoporosis in patients with the kidney Yin deficiency. We had also screened specific lincRNAs of POP kidney Yin deficiency using lincRNA chip. We used blat software to analyze the lincRNAs which targets to the CLCF1. The secondary structure of lincRNA was predicted using online software RNAfold. Patients with postmenopausal osteoporosis were randomly selected. Twenty-five patients were selected as kidney Yin deficiency syndrome group according to the TCM syndrome differentiation, and another 25 healthy postmenopausal women were selected as controls. The expression of CLCF1 mRNA and lincRNA uc431 + in the peripheral blood was detected using real-time RT-PCR. Results The CLCF1 was the target gene of lincRNA uc431 + according to the prediction of blat, with a correlation coefficient of -0, 8192 (P = 0. 0069). RNAfold software predicted that lincRNA uc431 + has several stem loop structure. The expression of CLCF1 mRNA in the kidney Yin deficiency group was significantly lower than that in the control group (P< 0. 01) . Compared with the control group, the expression of lincRNA uc431 + decreased in the the kidney Yin deficiency group (P <0.01). Conclusion Down-regulation of lincRNA uc431 + expression may involve in the pathogenesis of postmenopausal osteoporosis in patients with kidney Yin deficiency.