芦荟素通过激活ERK1/2-Runx 2信号通路介导对去卵巢大鼠骨量流失的保护作用

目的观察芦荟素对去卵巢大鼠骨量的影响,并探讨可能的机制。方法将30只雌性Sprague Dawley大鼠随机分为3组:假手术组(Sham)、去卵巢组(OVX)及去卵巢大鼠+芦荟素治疗组(LHS,去卵巢大鼠每天接受50 mg/kg芦荟素治疗,持续12周)。12周后取双侧股骨进行微型计算机断层扫描(Micro-CT)检测股骨干骺端微观结果,通过组织病理切片观察骨小梁结果变化,通过骨生物力学检测骨强度改变以及运用蛋白质印迹(WB)检测可能的机制。结果OVX组大鼠的骨密度(bone mineral density,BMD)、骨显微结构、最大载荷和弹性模量等指标均明显低于Sham组(P<0.05)。芦荟素治疗后大鼠的骨密度、骨显微结构、最大载荷和弹性模量等指标均有明显改善,比较差异有统计学意义(P<0.05)。WB检测显示OVX组p-Erk1/2、Erk1/2、ALP、RUNX 2、OCN和OPN表达水平较Sham组明显下调(P<0.05)。LHS组p-Erk1/2、Erk1/2、ALP、RUNX 2、OCN和OPN表达水平较OVX组明显上调。结论芦荟素可以显著改善去卵巢大鼠股骨骨强度和骨量,这种疗效可能通过激活ERK1/2-Runx 2信号通路促进成骨来实现的。

Aloin protects bone loss in ovariectomized rats by activating ERK1 / 2-Runx2 signaling pathway

Objective To observe the effect of aloin on the bone mass in ovariectomized rats and explore the possible mechanisms. Methods Thirty female Sprague Dawley rats were randomly divided into 3 groups: sham operation group (Sham); ovariectomized group (OVX) and ovariectomized rats + aloesin treatment group (LHS, ovariectomized rats received 50 mg / kg aloin for 12 weeks). After 12 weeks, the bilateral femurs were taken for micro-CT (Micro-CT), to detect the microscopic results of the femoral metaphysis, the changes in the results of trabecular bone were observed through histopathological sections, the changes in bone strength were detected through bone biomechanics, and the Western blot (WB ) was used to detect the possible mechanisms. Results The bone mineral density (BMD), bone microstructure, maximum load, and elastic modulus of the OVX group were significantly lower than those of the Sham group (P<0.05). After treatment with aloin, the bone density, bone microstructure, maximum load, and elastic modulus were significantly improved, which was statistically significant (P<0.05). WB detection showed that the expression levels of p-Erk1 / 2, Erk1 / 2, ALP, RUNX2, OCN and OPN in the OVX group were significantly lower than those in the Sham group (P<0.05). The expression levels of p-Erk1 / 2, Erk1 / 2, ALP, RUNX2, OCN and OPN in the LHS group were significantly higher than those in the OVX group. Conclusion Aloin can significantly improve the strength and bone mass of femurs in ovariectomized rats, and this effect may be achieved by activating the ERK1 / 2-Runx2 signaling pathway to promote osteogenesis.