辛伐他汀联合雷奈酸锶对成骨细胞及破骨细胞生物学特性的影响

目的探讨辛伐他汀(simvastatin,SIM)联合雷奈酸锶(strontium ranelate,SR)治疗对大鼠成骨细胞及破骨细胞生物学特性的影响。方法分离和培养大鼠成骨细胞和破骨细胞;使用SIM或SR和较低剂量SIM联合较低剂量SR处理大鼠成骨细胞和破骨细胞;用Western blot方法检测成骨细胞和破骨细胞中p-akt、p-gsk3β、β-catenin、p-β-catenin和NFATC1的蛋白水平。用相应的试剂盒测定ALP活性和TRACP活性。结果与单独使用SIM或SR相比,SIM+SR联合处理使成骨细胞活性明显增加,而破骨细胞分化和骨吸收能力明显降低(P<0.05)。进一步研究发现与单独使用SIM或SR相比,SIM+SR处理使成骨细胞中p-akt、p-gsk3β、β-catenin、p-β-catenin和NFATC1的蛋白水平显著增加(P<0.05);而破骨细胞中p-akt、p-gsk3β、β-catenin、p-β-catenin和NFATC1的蛋白水平显著降低(P<0.05)。结论较低剂量的SIM+SR比单独使用较高剂量的SIM或SR,更能显著通过AKT/GSK3β/β-catenin/NFATC1信号通路介导成骨细胞和破骨细胞功能。

Effects of simvastatin and strontium rainate on the biological characteristics of osteoblasts and osteoclasts

Objective To explore the effects of simvastatin(SIM)combined with strontium ranelate(SR)on the biological characteristics of osteoblasts and osteoclasts in rats.Methods The rat osteoblasts and osteoclasts were isolated and cultured,and rat osteoblasts and osteoclasts were treated with SIM or SR and lower dose SIM combined with strontium SR.The protein levels of p-akt,p-gsk3,β-catenin,p-β-catenin and NFATC1 in osteoblasts and osteoclasts were detected by Western blot method.The ALP activity and TRACP activity were determined by the corresponding kit.Results Compared with SIM or SR alone,the activity of osteoblasts was significantly increased,while the ability of osteoclast differentiation and bone resorption was significantly decreased by SIM and SR treatment.Further study showed that compared with SIM or SR alone,the protein levels of p-akt,p-gsk3,β-catenin,p-β-catenin and NFATC1 in osteoblasts treated with SIM and SR increased significantly(P<0.05).The protein levels of p-akt,p-gsk3,β-catenin,p-β-catenin and NFATC1 in osteoclasts decreased significantly.Conclusion Compared with high dose SIM or SR alone,lower dose SIM combined with SR significantly mediates the function of osteoblasts and osteoclasts through AKT/GSK3β/β-catenin/NFATC1 signaling pathway.