牛蒡苷元通过OPG/RANKL信号通路介导对去卵巢大鼠骨量流失的保护作用

目的探讨牛蒡苷元(AGN)对去卵巢大鼠骨强度和骨量的影响,并探索可能的机制。方法本研究中通过双侧去卵巢建立骨质疏松大鼠模型;随后随机分为假手术组(Sham)、去卵巢组(OVX)以及牛蒡苷元组(AGN),每组10只;其中牛蒡苷元组大鼠接受牛蒡苷元40 mg/(kg.d)]治疗12周;待治疗结束后使用Micro-CT、Masson染色切片、骨代谢指标、骨生物力学检测以及蛋白质印迹观察治疗效果以及可能的机制。结果治疗12周后,与OVX组相比,Micro-CT和Masson染色切片结果显示AGN组的大鼠骨小梁数量和骨密度得到明显改善。AGN组大鼠BMD、TV/BV、Tb.N、Tb.Th和Tb.Sp较OVX组明显改善(P0.05)。治疗12周时,AGN组极限载荷和刚度较OVX组显著增加(P0.05),而AGN组骨代谢指标AKP和TRACP水平显著降低(P0.05),差异有统计学意义(P0.05)。和OVX组比较,AGN组OPG表达水平明显上调,而RANKL表达水平显著下调,差异有统计学意义(P0.05)。表明AGN组的大鼠OPG/RANKL信号通路被激活。结论牛蒡苷元可以通过OPG/RANKL信号通路介导对去卵巢大鼠骨骼的保护作用。

Protection of Arctiin through OPG/RANKL signaling pathway on bone loss in ovariectomized rats

Objective To investigate the effect of arctiin (AGN) on bone strength and bone mass in ovariectomized rats and explore possible mechanisms. Methods In this study, osteoporosis rat models were established by bilateral ovariectomy; then they were randomly divided into sham operation group (Sham), ovariectomized group (OVX), and arctiin group (AGN), each group with 10 rats. The arctiin group rats were treated with arctiin (40mg / kg / day) for 12 weeks; after treatment, Micro-CT, Masson stained sections, bone metabolism indicators, bone biomechanical testing and Western blot were used to observe the treatment effect and possible Mechanisms. Results After 12 weeks of treatment, compared with the OVX group, the number of bone trabeculae and bone density in the AGN group were significantly improved. BMD, TV / BV, Tb.N, Tb.Th and Tb.Sp of rats in AGN group were significantly improved compared with those in OVX group (P<0.05). At 12 weeks of treatment, the limit load and stiffness of the AGN group were significantly increased compared to the OVX group (P<0.05), while the levels of bone metabolism indicators AKP and TRACP in the AGN group were significantly reduced (P<0.05), which was statistically significant (P<0.05). Compared with the OVX group, the expression level of OPG in the AGN group was significantly increased, while the expression level of RANKL was significantly decreased, and there was a statistical difference (P<0.05). This indicates that the OPG/RANKL signaling pathway in the AGN group was activated. Conclusion Arctiin can mediate the protective effect on the bones of ovariectomized rats through the activation of OPG / RANKL signaling pathway.