臭氧关节腔内注射治疗犬早期膝骨关节炎的最适低浓度

目的：通过建立家犬膝骨关节炎（osteoarthritis，OA）模型，比较3种低浓度医用臭氧关节腔内注射对0A治疗的最适浓度。方法健康雄性犬40只，随机分为A组（空气组）、B组（10 ug／ml医用臭氧组）、C组（20 ug／ml医用臭氧组）、D组（30 ug／ml医用臭氧组），每组10只，左膝关节10只为N组（正常对照组）。采用Hulth制模，分别于造模前、造模成功时和处死前测定膝关节活动度，用硝酸还原酶法检测关节液中TNF－α含量。结果8周后A组膝关节活动度显著降低，B、C、D组治疗结束后较造模成功时增加，但C组较B、D组活动度明显增加（ P＜0．05）；4组右侧膝关节均出现关节软骨病理损害， A组最严重，C组软骨病变最轻；关节液中TNF－α含量C组比A、B、D组明显降低，有统计学意义（P＜0．05）。结论20μg／ml臭氧组能够减轻炎症反应，增强机体清除自由基的能力，抑制TNF－α细胞因子表达，从而减轻退行性关节炎软骨退变。

Study of the optimal low concentration of ozone in the treatment of early knee osteoarthritis using intra-articular injection in dogs

Objective To compare the three low concentrationss of medical ozone for treating knee osteoarthritis ( OA) through intra-articular injection using the established knee OA model in dogs.Methods Forty healthy male dogs were randomly divided into Group A ( the air group) , Group B ( the 10 ug/ml medical ozone group) , Group C ( the 20 ug/ml medical ozone group) , and group D (the 30 ug/ml medical ozone group), with 10 dogs in each group.The left knee joints were selected as Group N (the normal control group) .Hulth method was used to create OA model.The range of knee motion was measured before modeling, successful modeling, and before execution of the dogs.Nitrale reduetase ( NR) method was used to detect the concentration of TNF-a in the joints.Results The range of knee motion in Group A decreased obviously after 8 weeks.The increased was more in Group B, C, and D after the therapy than that at the successful modeling.The range of knee motion in Group C increased more obviously than that in Group B and D (P<0.05).Articular cartilage pathological damage was found in the right knees of the dogs in 4 groups, and the most serious cartilage lesion was in Group A and the lest in group C.TNF-a concentration in Group C decrased more obviously than that in Group A, B, and D, which was of statistical difference ( P<0.05 ) .Conclusion The 20 ug/ml medical ozone can reduce inflammatory reaction, enhance the ability to eliminate free radicals of the body, and inhibit the expression of TNF-a.