牛磺酸防护高能冲击波致肾损伤的实验研究

目的 探讨牛磺酸对高能冲击波(HESW)致家兔肾损伤的防护作用及机制.方法20只雄性新西兰成年家兔随机分2组,每组10只,均予12.75 kV冲击波冲击左肾下极1500次.实验组在冲击的同时静脉滴注牛磺酸150 mg/kg,对照组给予等容积的生理盐水,在冲击前及冲击后3 d测定血丙二醛(MDA)、超氧化物歧化酶(SOD)的变化,检测肾脏组织学变化及热休克蛋白70(HSP70)的表达.结果 冲击前实验组和对照组血MDA分别为(2.2±0.4)、(2.1±0.4)mmol/L,SOD分别为(56.4±9.9)、(55.8±10.1)μU/L,差异均无统计学意义(P＞0.05).冲击后实验组血MDA、SOD分别为(2.7±0.7)mmol/L、(55.3±5.7)μU/L,与冲击前相比,差异无统计学意义(P＞0.05);对照组血MDA、SOD分别为(5.7±0.7)mmol/L、(33.3±3.5)μU/L,与冲击前相比,差异有统计学意义(P＜0.05).冲击后实验组和对照组肾小管评分分别为7.2±0.8和31.8±1.9,HSP70分别为25.2±4.1和6.4±0.9,差异均有统计学意义(P＜0.05).结论 牛磺酸对HESW致兔肾损伤有良好的防护作用,防护机制可能与其抗氧化作用和诱导HSP70合成增多有关.

Protective effects of taurine on renal injury induced by high energy shock wave

Objective To evaluate the protective effect of taurine on renal injury induced by high energy shock wave in rabbits.Methods Twenty rabbits were divided into the experimental (n=10)and the control group (n=10) at random.Both the groups were exposed to 1500 shock waves at 12.75 KV.In the experimental group, taurine (150 mg/kg) was given intravenously during the shock, while normal saline was given in the controls. Malondialdehyde(MDA) and superoxide dismutase(SOD) in serum were measured and the histological changes, the expression of heat shock protein 70(HSP70) in kidney were determined 3 days after the shocks.Results The level of MDA was(2.2±0.4) ,(2.1±0.4)mmol/L, and the level of SOD was (56.4±9.9) ,(55.8±10.1)μU/L respectively in the experimental and the control group before the shock.There was no significant difference between the two groups(P＞0. 05). After the shock, the level of MDA and SOD was (2.7±0.7)mmol/L, (55.3±5.7)μU/L respectively in the experimental group. There was no significant difference(P＞0.05) compared with the level of before the shock's. While in the control group, the level of MDA and SOD was (5. 7±0. 7) mmol/L, (33. 3±3. 5)μU/L respectively.There was significant difference (P＜0.05) compared with the level of before the shock's. The renal tubule scores of the experimental group and the control group after shocks were 7.2±0.8 and 31.8±1.9, respectively. And the HSP70 scores were 25.2±4.1 and 6.4±0.9, respectively. There was significant difference between the two groups (P＜0.05).Conclusions Taurine can effectively protect kidney from injury induced by high energy shock wave in rabbit.The mechanism may be related with its antioxidant capacity and inducing the synthesis of HSP70 in kidney.