结核分枝杆菌对材料粘附能力的体外实验研究

目的：通过体外实验观察结核分枝杆菌对内植物材料的粘附情况，从细菌粘附角度出发探讨脊柱结核内固定术的安全性问题，方法：以表皮葡萄球菌为对照，在表皮葡萄球菌、结核分枝杆菌菌液中分别加人不同材料，扫描电镜观察，比较不同细菌对不同材料(钛合金、不锈钢)、不同表面(光滑面、粗糙面)的粘附情况。结果：结核分枝杆菌对两种材料的粘附均较表皮葡萄球菌少，粗糙表面所粘附的细菌量较光滑面多。结论：结核分枝杆菌对内植物材料的低粘附性可能是临床脊柱结核内固定术安全的原因之一。

Study on adhesive properties of mycobacterium tuberculosis to implant material in vitro

Objective:To study the adhesion of Mycobacterium tuberculosis onto the surface of several implant materials and evaluate the risk of instrumentation surgery in spinal tuberculosis from the point of bacterial adhesion.Method:Staphylococcus epidermidis was selected as control group.Different material discs were individually placed in different bacterial suspensions.The adherences of Mycobacterium tuberculosis and Staphylococcus epidermidis onto the different surfaces(smooth?rough)of different material discs(titanium alloy?stainless steel)were investigated by scanning electron microscopy(SEM)respectively.Result:The adherence of Mycobacterium tuberculosis onto the surface of two materials was very few than that of Staphylococcus epidermidis.The amount of adhesive bacteria on rough surface were more than that on smooth surface.Conclusion:The adherence properties of Mycobacterium tuberculosis to implant material is poor.This characteristics maybe one reason for performing instrumentation surgery in spinal tuberculosis safely.