补肾活血中药对膝骨性关节炎家兔血清、滑膜及关节软骨一氧化氮水平的影响

目的探讨补肾活血中药对膝骨性关节炎家兔血清、关节软骨及滑膜一氧化氮(NO)水平的影响.方法 48只6～10个月龄新西兰大白兔,雌雄各半,随机分为正常组(18只),空白对照组(18只),中药组(12只).采用Hulth造模法建立OA模型.造模6周后分别给正常组和空白对照组、中药组每日灌服生理盐水、中药煎剂.分别于造模后6周、8周、12周测定各组血清、关节软骨及滑膜NO水平.结果空白对照组血清、关节软骨及滑膜NO水平均高于正常组,以关节软骨最为显著,而在灌服中药煎剂后,上述各项指标均有明显下降,与空白对照组比较有显著性差异(P＜0.05).结论补肾活血中药能够降低血清、关节软骨及滑膜NO水平,延缓OA的组织学改变进程,抑制本病的发生发展.

Experimental study of the effects of the level of the nitric oxide (NO) in serum,articular cartilage and syn- ovial membrane with the Chinese herbs of Busheng Huoxue in osteoarthritis rabbits knee

Objective To explore the effects of Busheng Huoxue Decoction on nitric oxide (NO) in serum,articular cartilage and synovial membrane in rabbits' knee joint Methods 48 New Zealand rabbits of 6- 10 months were used in this study. They were divided randomly into the normal group (18),control group (18) and Chinese herbs treating group (12).The rabbits in normal group underwent sham-operation,and others established the OA model with Hulth's method All rabbits, except the normal and control group, were given drugs from 6 weeks after operation. At 6 weeks,8 weeks and 12 weeks after operation, the NO concentration of the serum,articular cartilage and synovial membrane all of rabbits were examined. Results The NO concentration of the serum,articular cartilage and synovial membrane in control group was all significantly higher than those in normal group, with the articular cartilage more obvious than others'. Compared with other groups, the NO concentration of Chinese herbs treating group were obviously decreased (P < 0.05). But all of them were higher than that of the normal group. Conclusion The Busheng Huoxue Decoction was effective in preventing and treating osteoarthritis by means of decreasing the level of NO of the serum,synovial membrane and articalar cartilage.