Gene Expression of Leptin, Resistin, and Adiponectin in the White Adipose Tissue of Obese Patients with Non-Alcoholic Fatty Liver Disease and Insulin Resistance |
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| Authors: | Ancha Baranova Shobha J Gowder Karen Schlauch Hazem Elariny Rochelle Collantes Arian Afendy Janus P Ong Zachary Goodman Vikas Chandhoke Zobair M Younossi |
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| Institution: | (1) Center for Liver Diseases, Inova Fairfax Hospital, Washington, DC, USA; Center for the Study of Genomics in Liver Diseases, Molecular and Microbiology Department, George Mason University, Washington, DC, USA;(2) Center for the Study of Genomics in Liver Diseases, Molecular and Microbiology Department, George Mason University, Washington, DC, USA;(3) Center for Liver Diseases, Inova Fairfax Hospital, Washington, DC, USA;(4) Center for Liver Diseases, Inova Fairfax Hospital, Washington, DC, USA;(5) Center for Liver Diseases, Inova Fairfax Hospital, Washington, DC, USA; Center for the Study of Genomics in Liver Diseases, Molecular and Microbiology Department, George Mason University, Washington, DC, USA;(6) Center for Liver Diseases, Inova Fairfax Hospital, Washington, DC, USA;(7) Center for Liver Diseases, Inova Fairfax Hospital, Washington, DC, USA; Center for the Study of Genomics in Liver Diseases, Molecular and Microbiology Department, George Mason University, Washington, DC, USA;(8) Armed Forces Institute of Pathology, Washington, DC, USA;(9) Center for the Study of Genomics in Liver Diseases, Molecular and Microbiology Department, George Mason University, Washington, DC, USA;(10) Center for Liver Diseases, Inova Fairfax Hospital, Washington, DC, USA; Center for the Study of Genomics in Liver Diseases, Molecular and Microbiology Department, George Mason University, Washington, DC, USA |
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| Abstract: | Background: Adipose tissue is an active endocrine organ that secretes a variety of metabolically important substances including
adipokines. These factors affect insulin sensitivity and may represent a link between obesity, insulin resistance, type 2
diabetes (DM), and nonalcoholic fatty liver disease (NAFLD). This study uses real-time polymerase chain reaction (PCR) quantification
of mRNAs encoding adiponectin, leptin, and resistin on snap-frozen samples of intra-abdominal adipose tissue of morbidly obese
patients undergoing bariatric surgery. Methods: Morbidly obese patients undergoing bariatric surgery were studied. Patients
were classified into two groups: Group A (with insulin resistance) (N=11; glucose 149.84 ± 40.56 mg/dL; serum insulin 8.28
± 3.52 μU/mL), and Group B (without insulin resistance) (N=10; glucose 102.2 ± 8.43 mg/dL; serum insulin 3.431 ± 1.162 μU/mL).
Results: Adiponectin mRNA in intra-abdominal adipose tissue and serum adiponectin levels were significantly lower in Group
A compared to Group B patients (P<0.016 and P<0.03, respectively). Although serum resistin was higher in Group A than in Group B patients (P<0.005), resistin gene expression was not different between the two groups. Finally, for leptin, neither serum level nor gene
expression was different between the two groups. Serum adiponectin level was the only predictor of nonalcoholic steatohepatitis
(NASH) in this study (P=0.024). Conclusions: Obese patients with insulin resistance have decreased serum adiponectin and increased serum resistin.
Additionally, adiponectin gene expression is also decreased in the adipose tissue of these patients. This low level of adiponectin
expression may predispose patients to the progressive form of NAFLD or NASH. |
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| Keywords: | ADIPOSE TISSUE GENE EXPRESSION RESISTIN LEPTIN ADIPONECTIN MORBID OBESITY NON-ALCOHOLIC FATTY LIVER DISEASE |
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