胎羊单侧输尿管梗阻后肾脏病理相关研究

【摘要】〓目的〓探讨胎儿期发生单侧输尿管梗阻后,梗阻侧肾脏的病理变化过程。方法〓对孕75~85天的胎羊实行手术造成其单侧输尿管不完全性梗阻,在术后不同时期,取双侧肾脏(对侧肾脏作为对照),进行大体标本、组织学和分子学(PAX2和VEGF的表达)的检测。结果〓梗阻侧肾脏,表现为皮质变薄,皮质囊性改变、间质纤维化、肾小球数目减少;PAX2表达显著升高,而VEGF表达明显减少。结论〓在胎羊模型中,输尿管发生梗阻后,梗阻侧肾脏随之而来发生明显的病理变化。

Study of pathological lesions in kidneys of fetal lambs with unilateral partial ureteral obstruction

Objective To explore the dynamic changes of pathological lesions in the fetal kidney when the ureter was obstructed. Methods A silastic tube was tied around the left superior segment ureter to induce unilateral partial ureteral obstruction in fetal lambs at 75-85 days of gestation. In different postoperative stages, the kidneys of the fetal lambs were removed to study the changes in gross and histology,and the expression of paired-box 2 （PAX2）and vascular endothelial growth factor （VEGF） wene detected by immunohistochemistry assay. Results In the obstructed kidneys, we observed thinner renal cortex, cysts of various sizes in the cortex, fibrosis in the interstitial tissue, much decreased number of glomeruli, markedly increased PAX2 and decreased VEGF expressions. Conclusion In fetal lambs, when the ureter was obstructed, after the homolateral kidney gains markedly pathological changes.