Demonstration of 1,25(OH)2 vitamin D3 receptors and actions in vascular smooth muscle cellsIn vitro |
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Authors: | Dr J Merke W Hofmann D Goldschmidt E Ritz |
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Institution: | (1) Dept. Internal Medicine and Pathology, University of Heidelberg, Federal Republic of Germany |
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Abstract: | Summary Binding of 3H] 1,25 (OH)2D3 and effects of 1,25 (OH)2D3 on cell ultrastructure were evaluated in vascular smooth muscle cells (VSMC) primary cultures (aortic media). Specific reversible
binding of 3H] 1,25 (OH)2D3 by a 3.5 S macromolecule with DNA binding, KD 6.2×10−10M and Nmax 16 fmol/mg protein was demonstrated. Incubation of VSMC with 10−8 M 1,25 (OH)2D3, but not 25 (OH)D3, in the presence of 10% FCS for up to three weeks caused rapid reversible appearance in the cytoplasm of membrane-bounded
electron-dense lysosomal particles which on electronspectroscopic imaging contained Ca and Pi. VSMC are targets for vitamin
D. |
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Keywords: | vascular smooth muscle cells 1 25(OH)2 D3 receptors lysosomes atherogenesis hypertension |
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