FT-IR microscopy of endochondral ossification at 20μ spatial resolution |
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| Authors: | R Mendelsohn A Hassankhani E DiCarlo A Boskey |
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| Institution: | (1) Department of Chemistry, Rutgers Universit, Newark College of Arts and Science, Newark, New Jersey;(2) Department of Orthopaedic Pathology, Cornell University Medical College and The Hospital for Special Surgery, New York, New York, USA;(3) Department of Ultrastructural Biochemistry, Cornell University Medical College and the Hospital for Special Surgery, 10021 New York, NY, USA |
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| Abstract: | Summary A Fourier transform infrared spectrometer has been coupled with an optical microscope to study the distribution and characteristics
of the mineral phase in calcifying tissues at 20μ spatial resolution. This represents the first biophysical application of
this technique. High quality spectra were obtained in a relatively short scan time (1–2 minutes) from thin longitudinal sections
of normal and rachitic rat femurs. Substantial spatial variations in the extent and structure of the mineral phase were observed
as a function of spatial position both within and beyond the growth plates, as judged by the phosphate vibrations in the 900–1200
cm−1 spectral region. The current experiments reveal the utility of FT-IR micrscopy in identification of sites where mineralization
has occurred. In addition to vibrations from the inorganic components, the Amide I and Amide II motions of the protein constituents
are readily observed and may be useful as a probe of protein/mineral interactions. |
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| Keywords: | FT-IR FT-IR microscopy Endochondral ossification Infrared spectroscopy Hydroxyapatite |
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