| HIV-1假病毒包装方法的探讨 |
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| 引用本文： | 徐树莹,乔录新,史宣玲,徐萌,魏飞力,袁霖,石英,陈德喜.HIV-1假病毒包装方法的探讨[J].中华实验和临床感染病杂志（电子版）,2014(1):10-13. |
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| 作者姓名： | 徐树莹 乔录新 史宣玲 徐萌 魏飞力 袁霖 石英 陈德喜 |
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| 作者单位： | [1] 首都医科大学附属北京佑安医院感染科, 北京100069 [2] 首都医科大学附属北京佑安医院北京市肝病研究所 , 北京100069 [3] 清华大学医学部艾滋病综合研究中心, 北京100069 |
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| 基金项目： | 国家科技重大专项课题（No2012ZX10001002-002-011）;国家自然科学基金（No.30910103915）;国家自然科学基金青年基金（No.81100288） |
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| 摘 要： | 目的：通过不同方案包装HIV-1假病毒，确定获得高重复性、高稳定性和高滴度假病毒的方法。方法从转染效率影响因素出发，通过不同的转染方案，利用pNL4-3 LucR-E-质粒与VSV-G质粒共转染293细胞包装病毒，分别于48 h和72 h收取假病毒并重新感染293细胞，48 h后测荧光素酶报告基因。结果成功包装出HIV-1假病毒，不同包装方案的病毒滴度不同，测得报告基因最高接近107 RLUs。结论应用PEI转染试剂、大提质粒pNL4-3︰VSV-G为4︰1时，转染效率最高，包装的假病毒滴度最高。
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| 关 键 词： | 人类免疫缺陷病毒Ⅰ型 假病毒 包装方法 Human immunodeifciency virus typeⅠ |
Optimization of packaging the HIV-1 pseudotyped virus |
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| XU Shuying,QIAO Luxin,SHI Xuanling,XU Meng,WEI Feili,YUAN Lin,SHI Ying,CHEN Dexi.Optimization of packaging the HIV-1 pseudotyped virus[J].Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Version),2014(1):10-13. |
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| Authors: | XU Shuying QIAO Luxin SHI Xuanling XU Meng WEI Feili YUAN Lin SHI Ying CHEN Dexi |
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| Institution: | XU Shuying, QIAO Luxin, SHI Xuanling, XU Meng, WEI Feili, YUAN Lin, SHI Ying, CHEN Dexi |
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| Abstract: | Objective To package HIV-1 pseudotyped virus through different ways, to define methodology of packaging high reproductive, high stable and high titer pseudotyped virus. Methods In view of the transfection efifciency factors, through different transfection ways, pNL4-3 LucR -E -plasmid and VSV-G plasmid were transfected into 293 cells packaging virus, and the HIV-1 pseudotyped virus after 48 h and 72 h were collected, respectively, then reporter gene after infecting 293 cells for 48 h were detected. Results HIV-1 pseudotyped virus were packaged successfully, the virus titer differs from packaging ways, and reporter gene measured up to 107 RLUs. Conclusion When using PEI transfection reagent, and plasmid pNL4-3︰VSV-G as 4︰1, the highest transfection efifciency and the highest viral titer could received. |
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| Keywords: | Pseudotyped virus Packaging method |
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