胚肾发育过程中诱骗受体2表达与细胞衰老的关系

目的通过研究诱骗受体2(DcR2)在小鼠胚肾发育过程中的定位和表达，探讨DcR2在胚肾发育中与细胞衰老的关系。 方法分别选取胚龄为12.5 d、16.5 d、20.5 d和出生后8w小鼠的肾脏组织，使用过碘酸雪夫(PAS)染色观察肾组织形态，定量RT-PCR检测肾组织DcR2 mRNA表达水平，免疫组织化学染色观察DcR2的表达分布，免疫荧光共染检测DcR2与近端肾小管标志绒毛蛋白villin、远端肾小管标志水通道蛋白2(AQP-2)、衰老标志P16、胞核形态标志物核纤层蛋白B1(LaminB1)、增殖标志Ki-67和增殖细胞核抗原(PCNA)的共表达关系。 结果随着胚肾的发育，胚肾组织中DcR2 mRNA及蛋白表达逐渐增多，且明显高于成年肾脏；DcR2特异性表达于肾小管，且与villin共表达，但不与AQP-2共表达；DcR2阳性细胞高表达P16，却低表达LaminB1、Ki-67和PCNA。 结论DcR2特异性表达于胚肾近端肾小管细胞，且表达水平随胚龄增长而增多。此外，DcR2阳性细胞具有衰老相关表型，提示DcR2可能在胚肾发育过程中通过调控细胞衰老而具有重要作用。

Relationship between decoy receptor 2 expression and cellular senescence during embryonic kidney development

ObjectiveTo investigate the relationship between decoy receptor 2 (DcR2) and cellular senescence in embryonic kidney development by studying the localization and expression of DcR2 during the development of mouse embryonic kidney. MethodsKidney tissues of mice at the age of embryonic 12.5d, 16.5d, 20.5d, and postnatal 8w were selected respectively. Periodic acid Schiff (PAS) staining was used to observe the morphology of kidney tissues. Quantitative RT-PCR was used to detect the expression level of DcR2 mRNA. Immunohistochemical staining was used to observe the expression distribution of DcR2. And immunofluorescence co-staining was used to detect the co-expression relationship between DcR2 and proximal renal tubule marker villin, distal renal tubule marker aquaporin 2 (AQP-2), aging marker P16, nuclear morphological marker LaminB1, proliferation marker Ki-67, and proliferating cell nuclear antigen (PCNA). ResultsWith the development of the embryonic kidneys, the expression of DcR2 mRNA and protein in embryonic kidney tissues increased gradually, and were significantly higher than those in adult kidneys. DcR2 was specifically expressed in renal tubules, and co-expressed with villin, but not with AQP-2. DcR2-positive cells expressed high expression of P16, but low expression of LaminB1, Ki-67, and PCNA. ConclusionDcR2 was specifically expressed in embryonic kidney proximal tubular cells, and its expression level increased with embryonic age. In addition, DcR2-positive cells had cellular senescence-related phenotypes, suggesting that DcR2 may play an important role in regulating cellular senescence during embryonic kidney development.