FK506对肝移植术后血脂影响及高脂血症和GNB3基因C825T多态性的关系

目的 研究不同FK506血药浓度对肝移植术后血脂的影响及术后高脂血症发生与GNB3 C825T多态性的关系.方法 将58例乙肝后肝硬化肝移植患者依据FK506浓度分为高浓度（A组）和低浓度（B组）,统计患者肝移植术后1、3、6、12个月血清总胆固醇（TC）、甘油三脂（TG）的水平,评价FK506浓度差异对术后血脂的影响;并利用快速的序列特异性聚合酶链反应（SSP-PCR）方法,检测人GNB3 C825T单核苷酸多态性,分析GNB3 C825T多态性与肝移植FK506治疗后高血脂发生率的相关性.结果 两组患者组内术前与术后1年的TG水平差异有统计学意义（A组P＜ 0.001,B组P=0.049）,而TC水平差异无统计学意义（A组P=0.060,B组P=0.436）.两组术后1年的TG水平差异显著（P=0.002）,但两组高血脂发生率及TC水平差异不显著（P分别为0.207和0.317）,GNB3 C825T多态性与FK506治疗后高血脂发生率相关不显著（P=o.91）.结论 FK506影响肝移植术后TG的水平并与其浓度正相关;术后1年高脂血症发生率与FK506血药浓度不显著,与GNB3 C825T单核苷酸多态性相关也不显著.

Effect of FK506 on blood lipid after liver transplantation and relationship of GNB3 gene C825T polymorphism to serum lipid levels

Objective To study the relationship between C825T polymorphism in G protein beta 3 subunit （GNB3） gene and serum lipid levels in liver transplantations used different FK506 concentration.Methods Fifty-eight cases of patients after liver transplantation due to liver cirrhosis of hepatitis B were divided into two groups：high blood concentration group （group A） and low blood concentration group （group B）,based on the concentration of FK506,then the serum lipid levels in 1,3,6,12 months were detected.Genotype was determined by the simple sequence-specific primer polymerase chain reaction （SSP-PCR）.Results The serum triglyceride levels in group A and group B after one-year were significantly higher than that of the levels of preoperation respectively,but there was no difference in the serum cholesterol levels in group A and group B.There was a significant difference between two groups in the serum triglyceride levels but no difference in the serum cholesterol levels.The 825C/T polymorphism in the GNB3 gene and FK506 concentration was not associated with the incidence of hyperlipidemia after liver transplantation.Couclusion The serum triglyceride levels are affected by FK506 after liver transplantations,which is associated with the FK506 concentration.The 825C/T polymorphism in the GNB3 gene and FK506 concentration are not associated with the incidence of hyperlipidemia after liver transplantations.