卵子激活剂对无精子症患者冻融睾丸精子ICSI后妊娠结局的影响

目的研究卵子激活剂CultActive在临床中能否提高无精子症患者冻融睾丸精子行卵胞浆内单精子显微注射(ICSI)后的妊娠结局。方法选择2015年1月~2019年12月我院收治的188例行冻融睾丸精子ICSI助孕治疗的无精子症患者作为研究对象,按照患者ICSI后是否进行应用卵子激活剂CultActive随机分为冻融睾丸精子组(n=107)和卵子激活组(n=81),对照组为新鲜睾丸精子组(n=129),分别比较了三组患者在年龄、不孕年限、促性腺激素用量、促性腺激素使用天数等临床资料的差异以及获卵数、受精率、2PN率、分裂率、优胚率、可利用胚胎率、临床妊娠率等胚胎发育情况的差异。结果三组患者在年龄、不孕年限、促性腺激素用量、促性腺激素使用天数、获卵数上均无统计学差异(P>0.05)。B组在2PN率、优胚率、可利用胚胎率上均高于A、C组,但无统计学差异(P>0.05)。三组患者在分裂率上也无统计学差异(P>0.05)。B组在受精率上明显高于C组(P<0.05),且显著高于A组(p<0.01)。B组在临床妊娠率上显著高于A、C组(p<0.01)。结论卵子激活剂CultActive可以提高冻融睾丸精子ICSI效率,值得在临床中推广。

Effect of oocyte activator on pregnancy outcome after ICSI of frozen thawed testicular spermatozoa in azoospermia patients

Objective To study whether the oocyte activator CultActive can improve pregnancy outcome in patients with azoospermia after intracytoplasmic sperm microinjection(ICSI).Methods 188 patients with azoospermia treated with ICSI from January 2015 to September 2019 were selected as study subjects.According to whether the patients were treated with oocyte activator CultActive after ICSI,they were randomly divided into frozen thawed testicular sperm group(n=107)and oocyte activated group(n=81),while the control group was fresh testicular sperm group(n=129).The differences of clinical data,such as the age、infertile years、the dosage of gonadotropin and the days of gonadotropin use,as well as the differences of embryo development parameters,such as the number of obtained oocytes、fertilization rates、2 PN rates、cleavage rates、excellent embryo rates、available embryo rates、clinical pregnancy rates of the three groups were compared.Results There were not significantly differences among the three groups in age,infertile years,dosage of gonadotropin,days of gonadotropin used and the number of obtained oocytes(P>0.05).2 PN rates,quailty embryo rates and available embryo rates in group B were higher than those of group A and C.But there was not significantly differences(P>0.05).There was not significantly differences in the 2 PN division rate among the three groups of patients(P>0.05).The fertilization rate of group B was higher than that of group C(P<0.05)and significantly higher than that of group A(P<0.01).The clinical pregnancy rate of group B was significantly higher than that of group A and C(P<0.01).Conclusions The oocyte activator CultActive can improve the ICSI efficiency of frozen thawed testicular spermatozoa and was worth popularizing in clinic.