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金黄色葡萄球菌临床分离株功能基因及其二元分型研究
引用本文:翁幸鐾,糜祖煌.金黄色葡萄球菌临床分离株功能基因及其二元分型研究[J].中华临床感染病杂志,2014(1):21-26.
作者姓名:翁幸鐾  糜祖煌
作者单位:[1]浙江省宁波市第一医院检验科,315010 [2]无锡新区虎马生物信息工作室无锡市克隆遗传技术研究所生物信息学室,315010
摘    要:目的 研究一组金黄色葡萄球菌临床分离株毒力基因和耐药基因的存在状况.方法 连续收集浙江省宁波市第一医院2013年7至9月临床分离的金黄色葡萄球菌共40株,采用聚合酶链反应(PCR)的方法分析42种毒力基因和11种耐药基因,再以10类毒力基因和1种耐药基因mecA检测结果作二元分型.结果 40株金黄色葡萄球对青霉素的敏感率为12.5% (5/40),对红霉素的敏感率为42.5%(17/40),对其余15种抗菌药物的敏感率均大于65.0%.除了人主要组织相容性复合体(M HC)类似蛋白编码基因map未检出,其他几类毒力基因:黏附素、细胞毒素、荚膜抗原、超抗原、丝氨酸蛋白酶均有检出,检出率为2.5%~100.0%.耐β-内酰胺类、氨基糖苷类、红霉素类、四环素类、季铵盐消毒剂、抗菌肽的耐药基因均有检出,检出率为2.5%~37.5%.40株菌株经二元分型可分为16种阳性基因检出模式,每株菌最少检出3类毒力基因,最多检出7类毒力基因和1类耐药基因mecA.结论 本组菌株耐药表型和耐药基因型符合率较高,菌株携带多种毒力基因和耐药基因.

关 键 词:葡萄球菌  金黄色  毒力基因  耐药基因  二元分型

Binary typing of functional genes in clinical isolates of Staphylococcus aureus
Authors:Weng Xingbei  Mi Zuhuang
Institution:1.Department of Clinical Laboratory, Ningbo First Hospital, Ningbo 315010, Zhejiang Province,China;)
Abstract:Objective To investigate the distribution of virulence genes and resistance genes in a group of Staphylococcus aureus clinical isolates.Methods Forty strains of Staphylococcus aureus isolated from Ningbo First Hospital during July and September 2013 were collected.Forty-two kinds of virulence genes and 11 kinds of resistance genes were analyzed by polymerase chain reaction (PCR),and binary typing were performed based on 10 classes of virulence genes and resistance gene mecA.Results Among 40 Staphylococcus aureus strains,5 (12.5%) were sensitive to penicillin,and 17 (42.5%) were sensitive to erythromycin; The sensitive rates to the remaining 15 antibiotics were all higher than 65.0%.The positive rates of adhesins,cytotoxins,capsular antigens,superantigens,serine proteases were 2.5%-100.0%; While map gene was not detected.Resistance genes to β-lactam,aminoglycoside,erythromycin,tetracycline,polymer disinfectant and antibacterial peptide were also positive with positive rates of 2.5%-37.5%.By binary typing,40 strains of Staphylococcus aureus were divided into 16 kinds of positive modes.At least 3 classes of virulence genes were positive in all strains,and 7 classes of virulence genes and resistance gene mecA were positive in strain No.36.Conclusion The phenotypes of antibiotic resistance are well correlated with genotypes in this group of Staphylococcus aureus isolates,which carry several virulence genes and resistance genes.
Keywords:Staphylococcus aureus  Virulence gene  Resistance gene  Binary typing
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