胆汁酸对胆管癌细胞株QBC939中IL-6表达与细胞活力的影响

目的 探讨不同胆汁酸对胆管癌细胞株QBC939中IL-6的表达和细胞活力的影响.方法 分别使用不同浓度的游离胆汁酸以及其对应的甘氨酸结合型胆汁酸作用于胆管癌细胞株QBC939,药物浓度分别为:胆酸(CA)800 μmol/L、脱氧胆酸(DCA)100 μmol/L、鹅脱氧胆酸(CDCA)100 μmol/L、甘氨胆酸(GCA)1200 μmol/L、甘氨脱氧月胆酸(GDCA)200 μmol/L以及甘氨鹅脱氧胆酸(GCDCA)300 μmol/L.以MTT法检测不同胆汁酸刺激24、48、72 h后胆管癌细胞活力的变化,并以ELISA法检测肿瘤细胞中IL-6表达的改变.结果 DCA、CDCA、GCDCA作用48 h后,肿瘤细胞活力比值(A处理组/A对照组)分别为0.61、0.58和1.26;作用72 h后,CA、DCA、CDCA、GCA、GDCA和GCDCA各组肿瘤细胞活力比值分别为0.48、0.50、0.42、1.29、1.30和1.41;与对照组相比,以上各组细胞活力变化均具有统计学意义(P＜0.05).对照组肿瘤细胞培养48和72 h后,IL-6表达量分别为(198±32)ng/L和(323±34)ng/L,CA、DCA、CDCA、GCA、GDCA和GCDCA作用48 h后IL-6的表达量分别为(106±33)ng/L、(88±29)ng/L、(116±54)ng/L、(413±21)ng/L、(587±32)ng/L和(366±30)ng/L,作用72 h后IL-6表达量分别为(123±66)ng/L、(45±21)ng/L、(74±45)ng/L、(792±13)ng/L、(1310±22)ng/L和(845±18)ng/L;与对照组相比,以上各组IL-6表达量变化均具有统计学意义(P＜0.05).结论 游离胆汁酸CA、DCA和CDCA能减少胆管癌细胞IL-6的表达,并抑制细胞活力;而结合胆汁酸GCA、GDCA和GCDCA能增加胆管癌细胞IL-6的表达,并促进细胞活力.胆汁酸能通过IL-6途径改变胆管癌细胞活力.

Effects of bile acids on expression of interleukin-6 and cell viability in QBC939 cell line

Objective To research the effects of bile acids on the expression of interleukin-6 (IL-6)and the cell viability in QBC939 cell line. Methods Human cholangiocarcinoma cells were stimulated with 800 μmol/L bile acid (CA), 100 μmol/L deoxycholate (DCA), 100 μmol/L chenodeoxycholic acid (CDCA) ,1200 μmol/L gly acid (GCA) ,200 μmol/L glycodeoxycholic acid (GDCA) and 300 μmol/L gly chenodexycholic acid (GCDCA). MTT assay and ELISA were used to detect the cell viability and the expression of IL-6 at 24 h,48 h and 72 h. Results Treated by DCA, CDCA and GCDCA for 48 hours, the cell viability ratios changed to 0. 61,0. 58 and 1.26,which were significant differences between control group and treated groups. And after 72 hours, the viability ratios of group CA, group DCA, group CDCA, group GCA,group GDCA and group GCDCA turned into 0. 48,0. 50,0. 42,1.29,1.30 and 1.41. The differences of cell viability between bite acid-treated groups and control group were significant ( P ＜ 0. 05 ). The expression of IL-6 in control group at 48 h and 72 h was ( 198±32) ng/L and (323 ±34) ng/L,while treated by CA,DCA,CDCA,GCA,GDCA and GCDCA respectively for 48 hours,the expression of IL-6 altered to ( 106 ±33) ng/L,(88±29) ng/L,(116 ±54) ng/L,(413 ±21) ng/L,(587 ±32) ng/L and (366 ±30) ng/L.After 72 hours,the expression of IL-6 of each bile acid-treated groups as above was (123 ±66) ng/L, (45 ±21) ng/L,(74 ±45) ng/L,(792 ±13) ng/L,(1310 ±22) ng/L and (845 ±18) ng/L,respectively. The differences between each bile acid-treated group and control group were significant( P ＜0.05). Conclusions Free bile acids (CA, DCA and CDCA) can inhibit the expression of IL-6 and the cell viability, while glycineconjugates ( GCA, GDCA and GCDCA) can promote the expression of IL-6 and the cell viability. Bile acids can change tumor cell viability via IL-6 pathway.