神经生长因子加速猪深Ⅱ度烧伤创面愈合的实验

目的 探讨神经生长因子(nerve growth factors，NGF)对猪烧伤创面愈合的影响。方法 小白家猪6只，用控温控压仪在其背部制成24个直径为2．5cm的深Ⅱ度创面，随机分为4组，每6个创面为1组，即等渗盐水对照组，NGF1、2．5、5μg／ml治疗组。治疗后3、5、9d进行组织学检查、羟脯氨酸测定、细胞DNA周期分析及各组创面愈合时间比较。结果 治疗组上皮增生活跃且上皮化较对照组提前；治疗组创面组织羟脯氨酸含量，均有一个先下降后升高的过程，尤其在第5天，与对照组相比明显下降；细胞DNA周期分析表明，治疗组在S期细胞数较对照组均有明显增多；创面愈合时间较对照组提前。结论 局部应用NGF能促进烧伤深Ⅱ度创面愈合。

An experimental study on the effects of NGF on the wound healing of deep partial thickness burn in pigs

OBJECTIVE: To investigate the effects of nerve growth factor (NGF) on wound healing of deep partial thickness burn in pigs. METHODS: Six pigs of about 20 kg each were employed as the model. Twenty -- four deep partial thickness burn wounds (2.5 cm in diameter per wound) were made on the back of each pig by temperature and pressure controller. Six wounds were set to be one group. The wounds were randomly divided into 4 groups, i.e. control group (applied with normal saline, C), three treatment groups with NGF topically applied in doses of 1, 2.5 and 5 microgram/ml, respectively. Histological examination, determination of hydroxyproline, analysis of cellular DNA cycle and wound healing time in every groups were carried out on 3, 5 and 9 postburn days (PBD). RESULTS: The proliferation rate of epithelia was much more active in treatment groups than in control group. Epithelization of the wound in treatment groups was earlier than of that in control group. The hydroxyproline content in treatment groups exhibited an increment after a prior decrement, especially on 5 PBD when the content was much lower in the treatment groups. The analysis of cellular DNA cycle indicated that cell number of S phase in treatment groups was obviously more than that in the control group. Furthermore, the wound healing time in treatment groups was much shorter than that in control group. CONCLUSION: Local application of NGF might promote the wound healing of deep partial thickness burn.