长链非编码RNA CBR3-AS1在胆管癌中的表达及其临床意义

目的:探讨长链非编码RNA CBR3-AS1(lncRNA CBR3-AS1)在胆管癌(CCA)中表达状态及其临床意义。方法:用qRT-PCR检测lncRNA CBR3-AS1在CCA组织与癌旁组织以及CCA细胞与正常胆管上皮细胞中的表达,分析lncRNA CBR3-AS1表达与CCA患者临床病理参数及生存率的关系。将胆管癌细胞分别转染lncRNA CBR3-AS1过表达质粒(过表达组)、阴性对照质粒(对照组)及lncRNA CBR3-AS1沉默序列(沉默组),用MTT实验与Transwell实验检测各组细胞的增殖与侵袭能力。结果:lncRNA CBR3-AS1的表达在胆管癌组织中明显高于癌旁组织,在胆管癌细胞系中明显高于正常胆管上皮细胞(均P<0.01)。lncRNA CBR3-AS1表达与CCA患者淋巴结转移、TNM分期和术后复发明显有关(P<0.05)。lncRNA CBR3-AS1高表达患者总生存率明显低于lncRNA CBR3-AS1低表达患者(P=0.004)。lncRNA CBR3-AS1表达(P=0.020)与TNM分期(P=0.014)是影响CCA患者总生存率的独立危险因素。与对照组CCA细胞比较,过表达组CCA细胞增殖与侵袭能力明显增高,沉默组CCA细胞增殖与侵袭能力明显降低(均P<0.05)。结论:lncRNA CBR3-AS1在胆管癌中表达升高,升高的lncRNA CBR3-AS1与CCA的恶性临床病理特征及患者的不良预后密切相关。

Expression of long non-coding RNA CBR3-AS1 in cholangiocarcinoma and its clinical significance

Objective: To investigate the expression of long non-coding RNA CBR3-AS1 (lncRNA CBR3-AS1) in cholangiocarcinoma (CCA) and its clinical significance.  Methods: The expressions of lncRNA CBR3-AS1 in CCA tissue and tumor adjacent tissue as well as in CCA cells and normal biliary epithelial cells were determined by qRT-PCR. The relations of lncRNA CBR3-AS1 expression with the clinicopathologic variables and survival rates of the CCA patients were analyzed. The CCA cells were transfected with lncRNA CBR3-AS1 overexpression plasmid (overexpression group), negative control plasmid (control group) and lncRNA CBR3-AS1 silencing sequences (silencing group) respectively, and then, the proliferative and invasion abilities in each group of cells were examined by MTT and Transwell assay. Results: The lncRNA CBR3-AS1 expression in CCA tissue was significantly higher than that in tumor adjacent tissue, and in CCA cells was significantly higher than that in normal biliary epithelial cells (all P<0.05). The lncRNA CBR3-AS1 expression was significantly associated with the lymph node metastasis, TNM stage and recurrence of the CCA patients (all P<0.05). The overall survival rate in CCA patients with high lncRNA CBR3-AS1 expression was significantly lower than that in those with low lncRNA CBR3-AS1 expression (P=0.004). The lncRNA CBR3-AS1 expression (P=0.020) and TNM stage (P=0.014) were independent risk factors for overall survival rate of the CCA patients. Compared with the CCA cells in control group, the proliferative and invasion abilities were significantly increased in CCA cells in overexpression group, which were significantly reduced in CCA cells in silencing group (all P<0.05). Conclusion: LncRNA CBR3-AS1 expression is increased in cholangiocarcinoma, which is closely associated with the malignant features of CCA and poor prognosis of the patients.