Quercetin治疗裸鼠移植人肝癌的作用及机制

目的:探讨quercetin治疗裸鼠移植人肝癌中的作用及三磷酸肌醇( IP3) 和Bax基因表达变化。方法:采用quercetin治疗实验性肝癌,观察肝癌增长情况;用同位素试剂盒检测肝癌组织IP3 含量;RT-PCR分析癌组织bax mRNA的表达;Western blotting 分析肝癌组织bax蛋白的表达。结果:治疗组肝癌体积和重量均显著低于对照组［体积（15.8±10.1）mm3 vs. (52.3±26.5)mm3;重量（44.8±10.4）mg vs.(91.3±31.4) mg;均P<0.01］，IP3 含量显著低于对照组［（15.9±2.8）pmol/mg vs.（35.3±6.6）pmol/mg; 均P<0.01 ］，bax mRNA表达与对照组无显著性差异［RI（灰度与面积之积的相对强度）(0.64±0.12) vs.(0.56±0.15), P>0.05］，但bax蛋白表达显著高于对照组［RI( 3.16±0.95) vs.(1.37±0.48）］。结论:Quercetin能减少IP3 生成,上调肝癌组织bax蛋白的表达,抑制裸鼠移植人肝癌的增长。

Effect and mechanism of Querecetin treatment of transplanted hepatic cancer in nude mice

Abstract:Objective:To study the role of 1, 4, 5-trisphosphate inositol ( IP3) and bax gene expression in inhibiting transplanted hepatocellular carcinoma of nude mice by quercetin.Methods :After animals with hepatocellular carcinoma were treated with quercetin 1mg/kg/d (ip)for 3 weeks, the volume and weight of tumor was measured, and IP3, Bax mRNA, and Bax protein were assayed by IP3-［3H］ Birtrak assay,RT-PCR, and Western blotting, respectively; and compard with coutrol group.Results:The tumor volume and weight of animals treated with quercetin were lower than those of control［(15.8±10.1) mm3 vs. (52.3±26.5 mm3; (44.8±10.4) mg vs. (91.3±31.4) mg］，IP3 content was lower than that of control［（15.9±2.8）pmol/mg protein vs （35.3±6.6）pmol/mg protein］，Bax mRNA expression was not significantly different between the 2 groups［RI which was the gray degree multiply area of bax / the gray degree multiply area of β-actin (0.64±0.12) vs. (0.56±0.15)］，Bax mRNA expression was higher in group treated with quercetin than that of control［(3.16±0.95) vs. (1.37±0.48)］.Conclusions:Quercetin can inhibit growth of transplanted hepatocellular carcinoma of nude mice by reducing IP3 production and bax protein expression.