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| The in vitro myelin formation in neurospheres of human neural stem cells | |
| 作者姓名： | 杨立业 郑佳坤 刘相名 惠国桢 郭礼和 |
| 作者单位： | [1]DepartmentofNeurosurgery,ChaozhouCentralHospital,GuangdongProvince,Chaozhou521021,China [2]DepartmentofNeurosurgery,TheFirstHospitalofSoochowUniversity,Suzhou215006,China [3]InstituteofBiochemistryandCellBiology,ShanghaiInstituteforBiologicalSciences,ChineseAcademyofSciences,320YueyangRoad,Shanghai200031.China |
| 基金项目： | ThisworkwassupportedbytheNationalNaturalScienceFoundationofChina (KeyProjectNo.39993430 )andNaturalScienceFoundationofGuangdongProvince (No.0 12 4 5 2and0 2 0 0 0 1) |
| 摘 要： | Objective: To explore the culture conditions of human neural stem cells and to investigate the ultrastructure of neurospheres. Methods: The cells from the embryonic human cortices were mechanically dissociated. N2 medium was adapted to culture and expand the cells. The cells were identified by immunocytochemistry and EM was applied to examine the ultrastructure of neurospheres. Results: The neural stem cells from human embryonic brains were successfully cultured and formed typical neurospheres in suspension, and most of the cells expressed vimentin, which was a marker for neural progenitor cells, and the cells could differentiate into neurons, astrocytes and oligodendrocytes. In vitro myelin formation in neurospheres were observed at an early stageof culture. Conclusions: Human neural stem cells can be cultured from embryonic brains, can form the typical neurospheres in suspension in vitro and have the ability of myelinating, and may be potential source for transplantation in treating myelin disorders. |
| 关 键 词： | 髓磷酯 神经半球 神经干细胞 细胞培养 细胞移植 |
The in vitro myelin formation in neurospheres of human neural stem cells |
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| YANG Li-ye ,ZHENG Jia-kun,LIU Xiang-ming,HUI Guo-zhen and GUO Li-he.The in vitro myelin formation in neurospheres of human neural stem cells[J].Chinese Journal of Traumatology(English Edition),2003,6(6):341-345. | |
| Authors: | YANG Li-ye ZHENG Jia-kun LIU Xiang-ming HUI Guo-zhen and GUO Li-he |
| Institution: | 1. Department of Neurosurgery, Chaozhou Central Hospital, Guangdong Province, Chaozhou 521021, China 2. Department of Neurosurgery, The First Hospital of Soochow University, Suzhou 215006, China 3. Institute of Biochemistry and Cell Biology, Shanghai Institute for Biological Sciences, Chinese Academy of Sciences, 320 Yueyang Road, Shanghai 200031, China |
| Abstract: | Objective: To explore the culture conditions of human neural stem cells and to investigate the ultrastructure of neurospheres. Methods: The cells from the embryonic human cortices were mechanically dissociated. N2 medium was adapted to culture and expand the cells. The cells were identified by immunocytochemistry and EM was applied to examine the ultrastructure of neurospheres. Results: The neural stem cells from human embryonic brains were successfully cultured and formed typical neurospheres in suspension, and most of the cells expressed vimentin, which was a marker for neural progenitor cells, and the cells could differentiate into neurons, astrocytes and oligodendrocytes. In vitro myelin formation in neurospheres were observed at an early stage of culture. Conclusions: Human neural stem cells can be cultured from embryonic brains, can form the typical neurospheres in suspension in vitro and have the ability of myelinating, and may be potential source for transplantation in treating myelin disorders. |
| Keywords: | Transplantation Stem cells Culture media Myelin |
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