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绿脓杆菌制剂对人肝癌MHCC97L细胞增殖和侵袭能力的影响
引用本文:周建炜,黄修燕,居旻杰,汤钊猷,李涛,任正刚,刘彬彬.绿脓杆菌制剂对人肝癌MHCC97L细胞增殖和侵袭能力的影响[J].中华肝胆外科杂志,2009,16(10):455-459.
作者姓名:周建炜  黄修燕  居旻杰  汤钊猷  李涛  任正刚  刘彬彬
作者单位:河南省人民医院肿瘤内科,郑州,450003;复旦大学肝癌研究所、中山医院,教育部癌变与侵袭原理重点实验室,上海,200032;山东大学齐鲁医院普外科,济南,250012;
摘    要:Objective To investigate the effects of Pseudomonas aeruginosa vaccine (PA) on proliferation and invasiveness of the hepatocellular carcinoma cell line MHCC97L with metastatic potential. Methods Proliferation, growth curve, plate efficiency, flow cytometry, transwell invasion assay, cell motility assay, scarification test, vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP2) protein activity were evaluated after cells were treated with PA at various concentrations. Results PA can inhibit the proliferation and plate efficiency of MHCC97L cell markedly in a dose-dependent manner. The IC50 of cells treated with PA for 48 h and 72 h was 3.1 ×108/ml and 1.9 × 108/ml, respectively. The doubling time increased and plate efficiency decreased gradually when cells treated with 0.5 × 108/ml, 1 × 108/ml and 2 × 108/ml PA (P<0.01). PA could induce cell cycle arrest at the G1 phase in a dose-dependent manner by flow cytometric analysis. The average amount of invading cell per field in cell invasion assay and motility assay were 4. 8 ± 1.3 and 8. 8±2.2 when cells treated with 1× 108/ml PA, which was significantly lower than that of control group (8. 6±2. 1 and 15. 6±1.2 ) (P<0.01) Scarification test showed that the metastatic ability of cells treated with 1 × 108/ml PA significantly lower than that in the control group. Comparison between cells treated with 1 × 108/ml PA and control group, no remarkable difference was found regarding expression of VEGF and MMP2 in supernatant of cell culture. Conclusion PA can inhibit proliferation and plate efficiency of HCC cell line MHCC97L, which is in part mediated by the cell cycle arrest at the G1 phase. PA could inhibit invasiveness of HCC cell line MHCC97L, which is unrelated to the VEGF and MMP2 protein activity.

关 键 词:  肝细胞    绿脓杆菌制剂    细胞增殖    侵袭性    血管内皮生长因子    金属蛋白酶    

Effects of Pseudomonas aeruginosa vaccine on proliferation and invasiveness of hepatocellular carcinoma cell line MHCC97L
ZHOU Jian-wei,LI Tao,REN Zheng-gang,LIU Bin-bin,HUANG Xiu-yan,JU Min-jie,TANG Zhao-you.Effects of Pseudomonas aeruginosa vaccine on proliferation and invasiveness of hepatocellular carcinoma cell line MHCC97L[J].Chinese Journal of Hepatobiliary Surgery,2009,16(10):455-459.
Authors:ZHOU Jian-wei  LI Tao  REN Zheng-gang  LIU Bin-bin  HUANG Xiu-yan  JU Min-jie  TANG Zhao-you
Abstract:
Keywords:Carcinoma hepatocellularPseudomonas aeruginosa vaccineProliferationInvasivenessVEGFMMP2
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