结扎腹主动脉加双侧腹壁阴部动脉在大鼠后肢缺血模型中的应用

目的探讨结扎腹主动脉加双侧腹壁阴部动脉在SD大鼠后肢动脉缺血模型中的应用价值。方法将38只大鼠按随机数字表法随机分为假手术组(n=12)、结扎腹主动脉组(n=12)和结扎腹主动脉加双侧腹壁阴部动脉组(n=14)。氯胺酮6mg/100g腹腔注射麻醉大鼠,开腹后按各组不同的处理方法处理血管,观察术后2周和4周时各组后肢肌力、苍白等情况,同时取股静脉血做血气分析,并取后肢骨骼肌行HE染色。结果结扎腹主动脉加双侧腹壁阴部动脉组术后3d内死亡2只大鼠,其余大鼠均存活。假手术组术后无明显异常。结扎腹主动脉组术后立即出现后肢苍白、活动差;2周时后肢苍白、皮温低有好转;4周时恢复正常。结扎腹主动脉加双侧腹壁阴部动脉组术后2周时后肢缺血明显,肢体苍白、运动差;4周时仍有较明显的肌力异常、苍白等表现。各组均无后肢坏死。结扎腹主动脉加双侧腹壁阴部动脉组在术后2和4周时的肌力和苍白情况均较假手术组差(P0.05);与结扎腹主动脉组比较,在术后4周时肢体苍白情况仍较差(P0.05)。各组内不同时相间比较,差异均无统计学意义(P0.05)。结扎腹主动脉加双侧腹壁阴部动脉组的静脉血氧分压在术后2及4周时明显低于其他2组(P0.05)。HE染色:对照组显示正常的横纹肌结构;结扎腹主动脉组术后2周可见细胞染色不均,肿胀,横纹消失,部分细胞核染色深,4周时恢复正常;结扎腹主动脉加双侧腹壁阴部动脉组术后2周及4周见骨骼肌染色不均,细胞肿胀明显,横纹消失,部分细胞核染色深,密集,细胞间隙加大,但未见明显的细胞坏死。结论结扎腹主动脉加双侧腹壁阴部动脉可制作稳定的SD大鼠后肢缺血模型。

Application of Abdominal Aortic and Bilateral Abdominal Perineal Artery Ligation in Hindlimb Ischemia Model of Rats

Objective To investigate the application of abdominal aorta and bilateral abdominal perineal artery ligation in a hindlimb ischemia model of rats for research.Methods According to the random digits table,38 SD rats were divided into 3 groups randomly,sham operation group(SO group,n=12),abdominal aorta ligation group(AAL group,n=12)and abdominal aorta and bilateral abdominal perineal artery ligation group(AAL+BAPA group,n=14).Rats were anesthetized by 6 mg/100 g ketamine via intraperitoneal injection.Blood v...