Lgr5蛋白和E-钙黏蛋白在胃癌组织中表达的研究

目的检测胃癌组织中Lgr5及E-钙黏蛋白(E-cad)的蛋白表达情况并分析其与胃癌患者临床病理特征及预后的关系。方法采用免疫组织化学SABC方法检测69例胃癌组织及30例癌旁正常胃黏膜组织中Lgr5及E-cad的蛋白表达情况,同时分析其与胃癌患者临床病理特征和生存的关系。结果Lgr5和E-cad蛋白分别在60例(87.0%)和30例(43.5%)胃癌患者的胃癌组织中呈阳性表达,分别有5例(16.7%)和30例(100%)在癌旁正常胃黏膜组织中呈阳性表达,二者在不同组织中的蛋白表达阳性率比较差异均有统计学意义(Lgr5蛋白:χ^2=45.814,P<0.001;E-cad蛋白:χ^2=11.249,P=0.001)。Lgr5和E-cad蛋白在胃癌组织中的阳性表达均与分化程度和浸润深度有关(P<0.05),Lgr5蛋白在胃癌组织中的阳性表达还与淋巴结转移和幽门螺杆菌(HP)感染有关(P<0.05)而E-cad蛋白阳性表达却与此无关(P>0.05)。Lgr5蛋白表达阳性和阴性患者的5年总生存情况比较差异无统计学意义(χ^2=1.819,P=0.117),而E-cad蛋白表达阳性胃癌患者的5年总生存情况明显优于E-cad蛋白表达阴性胃癌患者(χ^2=5.814,P=0.016)。Lgr5蛋白表达与E-cad蛋白表达呈明显的负相关(rs=-0.355,P=0.003)。结论Lgr5蛋白可能参与了肿瘤组织局部浸润、淋巴结转移过程,与肿瘤恶生程度有关,未发现其阳性表达与预后有关;E-cad的表达可能参与了肿瘤的肿瘤局部侵袭和进展且与患者预后有关。

Research of expressions of E-cadherin and Lgr5 proteins in gastric cancer tissues

Objective To detect expressions of Lgr5 and E-cadherin(E-cad)proteins in gastric cancer tissues and analyze their relationships with the clinicopathologic characteristics and prognosis of patients with gastric cancer.Methods The expressions of Lgr5 and E-cad proteins in the 69 patients with gastric cancer and adjacent normal gastric mucosa tissues were measured by the immunohistochemical SABC method,and the relationships between the Lgr5 or E-cad protein expression in the gastric cancer tissues and the clinicopathologic characteristics and the survival of patients with gastric cancer were analyzed.Results The expressions of Lgr5 and E-cad proteins were positive in 60 cases(87.0%)and 30 cases(43.5%)of gastric cancer tissues,respectively,and in 5 cases(16.7%)and 30 cases(100%)of adjacent normal gastric mucosa tissues.There was a significant difference in the positive rate of Lgr5 or E-cad protein expression in the different tissues,respectively(Lgr5 protein:χ^2=45.814,P<0.001;E-cad protein:χ^2=11.249,P=0.001).The positive rates of Lgr5 and E-cad protein expressions in the gastric cancer were related to the degree of differentiation and the depth of invasion.Meanwhile the positive rate of Lgr5 protein expression in the gastric cancer tissue was also related to the lymph node metastasis and Helicobacter pylori infection,while the positive rate of E-cad protein expression was not related to these(P>0.05).The 5-year total survival time had no significant difference in the patients between with positive and with negative expressions of Lgr5 protein(χ^2=1.819,P=0.117),which had a significant difference in the patients between with positive and with negative expressions of E-cad protein(χ^2=5.814,P=0.016).The positive expression of Lgr5 was negatively correlated with that of E-cad(rs=?0.355,P=0.003).Conclusions Lgr5 protein may get involved in the mechanism of tumor invasion,lymph nodal metastasis,and low differentiation,while no relationship between the Lgr5 protein and prognosis has been confirmed.E-cad protein may get involved in the mechanism of tumor invasion and affect the prognosis of patients.