果糖在微囊化肝细胞制作过程中的细胞保护作用

目的 为了减少肝细胞在微囊化过程中的缺氧损伤，研究在缺氧状态下果糖对肝细胞的保护作用。方法 将ＳＤ大鼠分为三组；Ａ组，细胞分离的灌流液和消化液中均加果糖２０ｍｍｏｌ／Ｌ，Ｂ，Ｃ两组不加果糖；细胞分离及微囊化后，检测细胞的活率，微囊化肝细胞体外培养，Ｃ组培养液中加果糖１０ｍｍｏｌ／Ｌ，Ａ，Ｂ两组不加。

The protective effect of fructose in the process of microencapsulation of hepatocytes

Objective To study the protective effect of fructose with regard to hypoxia induced hepatocytes injury during the long microencapsulation periods. Methods Eighteen SD rats were divided into three groups, 6 rats each. In group A, fructose was added into the perfusion solutions (20?mmol/L). There was no fructose in group B and C. After isolation and microencapsulation, the viability was tested. In vitro culture, fructose (10?mmol/L) was added in culture medium in group C, but not in groups A and B. LDH leakage and TP of culture were examined.Results The survival of hepatocytes in group A was obviously higher than in groups B and C ( P < 0.01 ). The LDH leakage in group A was significantly lower than in groups B and C ( P < 0.01 ).Conclusion Fructose could protect hepatocytes against hypoxia induced cell damage during microencapsulation, but cells should be pre treated with fructose before hypoxia.