供者应用粒细胞集落刺激因子促进异基因骨髓移植受者造血重建的临床研究

目的评价供者应用粒细胞集落刺激因子（G-CSF）以增加骨髓中CD34+细胞的效果和对异基因骨髓移植造血重建的影响。方法供者采髓前应用G-CSF250μg/d，连用7d，随后对15例白血病患者进行异基因骨髓移植，观察植入物单个核细胞、CD3

The clinical study on accelerating hemopoietic reconstitution of allo-bone marrow transplantation recipients by using G-CSF in donors

Objective　To investigate the effects of donor administered G-CSF in accelerating CD34+ cells in bone marrow and on hemopoietic reconstruction in allo-bone marrow transplantation (BMT). Methods　Fifteen consecutive patients with leukemia who underwent allo-BMT infused marrow stimulated with G-CSF 250!μg/d for 7 days. The changes in the number of mononuclear cells, CD3+ cells, CD34+ cells and CFU-GM infused marrow cells in the grafts and hemopoietic recovery after BMT were estimated. The changes of CD34+ cells before and after administration of G-CSF in 15 donors and the changes in hematopoetic progenitor cells after treatment with G-CSF in 3 donors were determined. Results　Hematopoietic reconstruction after allo-BMT in 15 patients were accelerated. The days of leukocyte count exceeding 1.0×109/L and platelet count exceeding 20×109/L were (16.1±3.1)days and (18.4±4.0)days respectively (26.2±6.6 and 28.1±7.1 days respectively in the control group, P＜0.01). CD34+ cells and CFU-GM in the grafts were obviously increased as compared with those in the control group (P<0.01). CD34+ cells were increased both before and after stimulation of G-CSF, CFU-GM and CFU-MK were vigorously proliferated, especially the proliferation of CFU-GM. Conclusions　CD34+-enriched marrow cells infused with G-CSF stimulating donor BM might be a reliable indicator of accelerating hemato-poietic recovery. The elevation in number of CFU-MK was associated with accelerated platelet recovery.