人工毛乳头异种移植诱导大鼠足垫毛囊形成

目的观察人头皮毛乳头细胞微囊(人工毛乳头)异种移植诱导大鼠足垫毛囊形成的能力。方法以海藻酸钠-多聚赖氨酸-海藻酸钠(alginate-polylysine-alginate,APA)微囊包裹分离培养的毛乳头细胞;对体外培养1、4周的毛乳头细胞微囊及无APA的微囊对照组行组织学观察;取培养4周的毛乳头细胞微囊移植至大鼠足垫皮下,6周后取材行组织学检查。结果毛乳头细胞微囊体外培养1周后,毛乳头细胞周围出现细胞外基质;4周后,囊中形成“类毛乳头样结构”;人头皮毛乳头细胞微囊移植至大鼠足垫6周后,移植部位及其周围皮下有大量毛囊及皮脂腺结构形成。结论人工毛乳头诱导并参与了无毛区域新生毛囊及皮脂腺的组织构成。

Hair follicles induced by microencapsulated human hair dermal papilla cells in rat footpad

Objective To induce the hair follicle formation in SD rat footpads by microencapsulated human hair dermal papilla cells (reconstructed dermal papilla). Methods The dermal papilla cells were encapsulated with alginate-polylysine-alginate by using a high-voltage electric field droplet generator. After 1-4 weeks culture, the histological examination was performed in both of reconstructed dermal papilla(DP) and empty microcapsulation. The reconstructed DP were xenotransplanted into the SD rat footpads. The histological examination was detected by microscopy after 6 weeks. Results Extracellular matrix was found around the dermal papilla cells after 1 week culture. Dermal papilla-like structure was found inside the microcapsule after 4 weeks cultrue. At 6 weeks following xenotransplantation, large numbers of fully developed hair follicles were emerged in SD rat footpad. Conclusion The reconstructed DP induced and participated in the formation of regenerated hair follicles.