瘢痕疙瘩成纤维细胞增殖和凋亡的研究

目的　探讨瘢痕疙瘩的成纤维细胞的增殖和凋亡状态。方法　采用免疫组化和末端脱氧核苷酸转移酶介导的末端标记技术 ,检测 2 4例瘢痕疙瘩和其周围正常皮肤中成纤维细胞的增殖和凋亡状态。以每 10个高倍镜(40 0倍 )视野中成纤维细胞凋亡及PCNA阳性细胞数与总细胞数之比作为凋亡指数和增殖指数 ,利用配对t检验进行统计学处理。结果　 2 4例瘢痕疙瘩成纤维细胞和对照组平均增殖指数、平均凋亡指数、平均凋亡指数 /平均增殖指数 ,经配对t检验具有高度显著性差别。结论　瘢痕疙瘩成纤维细胞的过量增生和凋亡相对减少在瘢痕疙瘩的发生和发展中起着重要作用。

Investigation of proliferative and apoptotic status in keloid fibroblast

Objective To investigate proliferative and apoptotic status of fibroblasts in keloid development. Methods Proliferative status of keloid fibroblasts and normal skin around it were investigated in 24 cases by immunohistochemical analysis of PCNA and apoptotic status by TUNEL method. Results Nuclei of PCAN positive cell in proliferative and apoptotic investigation were brown and had minute pellets.The rate of PI, AI and AI/PI of fibroblasts in keloid and normal skin showed statistical differences. Conclusion High fibroblasts proliferating rate and relatively lower apoptotic rate play a key role in keloid development.