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精液中性激素水平与生殖细胞凋亡的关系
引用本文:杨麦贵,杨阳,黄萍,郝晓柯,张竹映,郑善銮,樊爱琳,饶国洲,韦晓明.精液中性激素水平与生殖细胞凋亡的关系[J].中华男科学杂志,2006,12(5):432-434.
作者姓名:杨麦贵  杨阳  黄萍  郝晓柯  张竹映  郑善銮  樊爱琳  饶国洲  韦晓明
作者单位:1. 第四军医大学,西京医院检验科,陕西,西安,710032
2. 第四军医大学,学员旅,陕西,西安,710032
3. 第四军医大学,秦都口腔医院检验科,陕西,西安,710032
4. 中国铁道建筑总公司西安卫校,陕西,西安,710016
5. 西安交通大学口腔医学院中心实验室,陕西,西安,710004
基金项目:第四军医大学校科研和校改项目
摘    要:目的:探讨人精液中性激素水平与生殖细胞凋亡的关系。方法:随机选择66例不育患者及30例生育男性,采用ELISA法进行卵泡刺激素(FSH)、黄体生成素(LH)、泌乳素(PRL)和睾酮(T)水平分析,并用脱氧核苷酸末端转移酶(TdT)介导的缺口末端标记(TUNEL)法检测生殖细胞的凋亡。结果:精液中FSH、LH、PRL和T水平30例生育者分别为(1.63±0.15)U/L、(2.18±0.21)U/L、(6.34±0.30)nmol/L、(1.85±0.11)nmol/L,生殖细胞凋亡率为(4.61±1.23)%,而66例不育者4种性激素水平分别为(1.25±0.18)U/L、(1.76±0.32)U/L、(5.86±0.31)nmol/L、(1.45±0.13)nmol/L,生殖细胞凋亡率为(18.36±2.04)%。两者相比各参数差异均有显著性(P<0.01)。不育组FSH、LH、PRL、T水平与生殖细胞的凋亡率呈负相关(r分别为-0.88、-0.93、-0.90、-0.98,P均<0.01)。凋亡的生殖细胞体积缩小,核染色质致密,凝聚在核周形成新月形,或核裂解形成凋亡小体。结论:精液性激素水平与生殖细胞的凋亡有着密切关系,低水平的性激素可能导致睾丸生殖细胞凋亡率增加。

关 键 词:性激素  生殖细胞  凋亡  精液
文章编号:1009-3591(2006)05-0432-03
收稿时间:2005-04-21
修稿时间:2005-08-01

Sexual Hormone Levels in Semen and Germ Cell Apoptosis
YANG Mai-gui,YANG Yang,HUANG Ping,HAO Xiao-ke,ZHANG Zhu-ying,ZHENG Shan-luan,FAN Ai-lin,RAO Guo-zhou,WEI Xiao-ming.Sexual Hormone Levels in Semen and Germ Cell Apoptosis[J].National Journal of Andrology,2006,12(5):432-434.
Authors:YANG Mai-gui  YANG Yang  HUANG Ping  HAO Xiao-ke  ZHANG Zhu-ying  ZHENG Shan-luan  FAN Ai-lin  RAO Guo-zhou  WEI Xiao-ming
Institution:Department of Clinical Laboratory, Xijing Hospital, Xi'an, Shaanxi 710032, China. YangMg@fmmu.edu.cn
Abstract:OBJECTIVE: To explore the relationship between sexual hormones in semen and germ cell apoptosis in male population. METHODS: Sixty-six infertile patients and thirty fertile males were selected randomly. The levels of folicle stimulating hormone ( FSH), prolactin (PRL), luteinizing hormone (LH), and testosterone (T) in semen were measured by ELISA. Terminal deoxynucleotidyl transferase mediated UTP nick end labeling (TUNEL) was used for the detection of germ cell apoptosis. RESULTS: The levels of FSH, LH, PRL, T in thirty fertile men were (1.63 +/- 0.15) U/L, (2.18 +/- 0.21) U/L, (6.34 +/- 0.30) nmol/L, (1.85 +/- 0.11) nmol/L, respectively, and germ cell apoptosis rate was (4.61 +/- 1.23)%. FSH, LH, PRL, T levels in infertile group were (1.25 +/- 0.18) U/L, (1.76 +/- 0.32) U/L, (5.86 +/- 0.13) nmol/l, (1.45 +/- 0.13) nmol/, respectively, and germ cell apoptosis rate was (18.36 +/- 2.04)%. There were significant differences in all parameters between infertile group and fertile group. The levels of FSH, LH, PRL, T were negatively correlated with germ cell apoptosis rates( r = -0.88, -0.93, -0.90, -0.98). The volume of apoptotic germ cell decreased, and chromatin was compacted to form cell-membrane blebs and apoptotic bodies. CONCLUSION: Low concentration of sexual hormones may increase the apoptosis of germ cells, which can induce male infertility.
Keywords:sexual hormone  germ cell  apoptosis  semen
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