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镉离子对公牛精子功能损伤的机制
引用本文:Mehran ARABI Mohammad Saied HEYDARNEJAD 吕年青.镉离子对公牛精子功能损伤的机制[J].中华男科学杂志,2007,13(4):291-296.
作者姓名:Mehran  ARABI  Mohammad  Saied  HEYDARNEJAD  吕年青
作者单位:[1]Department of Biology, Shahrekord University, Shahrekord, Iran [2]不详, Shahrekord University, Shahrekord, Iran
基金项目:This work was partly supported by Pazhoohesh section in Shahrekord University, Iran.
摘    要:目的:重金属比如镉(Cd)作为工业污染物广泛分布在环境中,研究人员已经鉴定了这些污染物能影响男性生殖系统。本文研究的目的是测试Cd在10~1000μmol/L的浓度范围在体外对荷斯坦(Holstein)公牛精子膜和DNA完整性、活动率和精子顶体胞吐能力的影响。方法:用PBS处理公牛精液样本后进行精液分析。脂质过氧化试验评估精子膜完整性。明胶消化试验测定公牛精子顶体胞吐作用的能力。单细胞凝胶电泳(SCGE)检测单个细胞内DNA断裂和不耐碱的破坏。结果:脂质过氧化(LPO)显著增加,表明了Cd对精子膜完整性的破坏作用。这种影响在Cd浓度为1000μmol/L时特别明显。LPO与活动精子百分率之间呈负相关(r=-0.94,P<0.001)。明胶消化试验表明Cd引起公牛精子顶体胞吐作用的百分率下降。发现在LPO率和消化环百分率之间呈负相关(r=-0.97,P<0.001)。彗星试验获得的数据表明Cd能诱导精子核中的DNA断裂。接近93%的DNA损伤为双股断裂。LPO氧化率与DNA断裂百分率之间的相关性为0.95(P<0.001)。结论:总体上,Cd诱导公牛精子膜损伤、活动率降低、DNA断裂、以及顶体反应率降低而导致精子功能损伤。进入雄性性腺和精浆的Cd可能对动物精子产生了破坏作用。

关 键 词:顶体反应  公牛精子  镉离子  DNA断裂  脂质过氧化  活动率
文章编号:1009-3591(2007)04-0291-06
收稿时间:2007-01-17
修稿时间:2007年1月17日

Mechanism of the Dysfunction of the Bull Spermatozoa Treated with Cadmium
Mehran ARABI,Mohammad Saied HEYDARNEJAD.Mechanism of the Dysfunction of the Bull Spermatozoa Treated with Cadmium[J].National Journal of Andrology,2007,13(4):291-296.
Authors:Mehran ARABI  Mohammad Saied HEYDARNEJAD
Institution:Department of Biology, Shahrekord University, Shahrekord, Iran
Abstract:OBJECTIVE: Heavy metals such as cadmium (Cd) are widely distributed in the environment as industrial pollutants and characterized by their ability to affect the male reproductive system. The objective of the present study was to test the effect of Cd in the concentration range from 10 to 1000 micromol/L, in vitro, on the membrane and DNA integrity, motility, and ability of sperm to undergo acrosomal exocytosis in Holstein bull spermatozoa. METHODS: Bull semen samples were processed for sperm analyses using semen-diluting fluid, PBS. Membrane integrity of the processed bull sperm was evaluated by lipoperoxidation (LPO) test. Gelatin digestion test was performed to determine the ability of bull spermatozoa to undergo acrosomal exocytosis. Single cell gel electrophoresis (SCGE) assay was performed to detect the DNA strand breaks and alkali labile damages in the individual cell. RESULTS: We found a significant increase in the lipoperoxidation (LPO) indicating the deleterious effect of Cd on the sperm membrane integrity. This effect was prominent at the concentration of 1000 micromol/L Cd. There was a negative correlation between LPO rate and the percentage of motile spermatozoa (r = -0.94, P < 0.001). The gelatin digestion test indicated that Cd caused a decline in the percentage of acrosomal exocytosis of bull spermatozoa. A reverse correlation was also found between LPO rate and the percentage of halos (r = -0.97, P < 0.001). Data obtained from the comet assay revealed that Cd was capable of inducing DNA breaks in the sperm nuclei. Almost 93% of DNA damages were double-stranded breaks. The correlation between LPO rate and the percentage of DNA breaks was found to be 0.95 (P < 0.001). CONcLUSION: Collectively, Cd induced membrane impairments, lowered motility, DNA breaks and a decreased rate in the acrosome reaction of bull spermatozoa, leading to sperm dysfunction. Entering Cd in the male gonads and seminal plasma may exert deleterious effects on the animal sperm cells.
Keywords:acrosome reaction  bull sperm  cadmium  DNA break  lipoperoxidation  motility
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